Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 805, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2021.149930
Keywords
SARS-CoV-2; COVID-19; Virus; Wastewater; Sewage; Mutation; Variant
Categories
Funding
- NIH [GM121325, GM103440, MH109706]
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Monitoring wastewater from dormitories can effectively identify viral genomes, benefiting COVID-19 wastewater surveillance programs.
In the Fall of 2020, university campuses in the United States resumed on-campus instruction and implemented wastewater monitoring for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While quantitative polymerase chain reaction (qPCR) tests were deployed successfully to detect viral RNA in wastewater across campuses, the feasibility of detecting viral variants from a residential building like a dormitory was unclear. Here, we demonstrate that wastewater surveillance from a dormitory with at least three infected students could lead to the identification of viral genomes with more than 95% coverage. Our results indicate that viral variant detection from wastewater is achievable at a dormitory and that coronavirus disease 2019 (COVID-19) wastewater surveillance programs will benefit from the implementation of viral whole genome sequencing at universities. (c) 2021 Elsevier B.V. All rights reserved.
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