4.7 Article

SARS-CoV-2 detection in wastewater using multiplex quantitative PCR

Related references

Note: Only part of the references are listed.
Editorial Material Immunology

Concomitant Marked Decline in Prevalence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and Other Respiratory Viruses Among Symptomatic Patients Following Public Health Interventions in Australia: Data from St Vincent's Hospital and Associated Screening Clinics, Sydney, NSW

Deborah Marriott et al.

Summary: According to a study conducted in an Australian hospital, the number of diagnosed cases of SARS-CoV-2 and other respiratory viruses significantly decreased after travel bans and social distancing measures were put in place. However, an increase in rhinovirus diagnoses after the relaxation of social control measures may indirectly indicate an elevated risk of COVID-19 resurgence.

CLINICAL INFECTIOUS DISEASES (2021)

Article Immunology

Multiplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assays for Diagnostic Testing of Severe Acute Respiratory Syndrome Coronavirus 2 and Seasonal Influenza Viruses: A Challenge of the Phase 3 Pandemic Setting

Fabiola Mancini et al.

Summary: The study demonstrates that the multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay is a rapid, valid, and accurate method for detecting SARS-CoV-2 and influenza viruses in clinical samples. This test is able to distinguish different virus targets, detect coinfections, and serve as an important diagnostic and surveillance tool during seasonal influenza activity.

JOURNAL OF INFECTIOUS DISEASES (2021)

Review Environmental Sciences

Coronavirus (SARS-CoV-2) in the environment: Occurrence, persistence, analysis in aquatic systems and possible management

Manvendra Patel et al.

Summary: The emergence of COVID-19 in 2020 has become a global pandemic, with various sources of viral transmission. Recently, targeted SARS-CoV-2 genome fragments have been detected around the world.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Medical Laboratory Technology

Assessment of Multiplex Digital Droplet RT-PCR as a Diagnostic Tool for SARS-CoV-2 Detection in Nasopharyngeal Swabs and Saliva Samples

Kevin Cassinari et al.

Summary: The newly developed multiplex RT-ddPCR assay showed higher sensitivity in COVID-19 diagnosis compared to traditional methods, especially in saliva samples. It can serve as a complementary tool to control ambiguous results from RT-qPCR and enable repetitive sampling with saliva when nasopharyngeal swabbing is not possible.

CLINICAL CHEMISTRY (2021)

Article Pathology

Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexing

Raphael Nyaruaba et al.

Summary: This study demonstrates the development of different ddPCR SAR-CoV-2 assays, including multiplex assays. Results show that the quadruplex assay has similar detection limits and accuracy to lower multiplex assays, and the triplex probe mix assay has better sensitivity than the RT-qPCR assay. Additionally, the ddPCR multiplex assay indicates remdesivir's ability to inhibit SARS-CoV-2 growth in vitro.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021)

Article Microbiology

Clinical evaluation of a fully automated, laboratory- developed multiplex RT-PCR assay integrating dual- target SARS-CoV-2 and influenza A/B detection on a high- throughput platform

Dominik Noerz et al.

Summary: This study developed a laboratory-developed multiplex RT-PCR assay for simultaneous detection of SARSCoV-2, influenza A, and influenza B, demonstrating comparable analytical performance to current commercial tests and excellent agreement in clinical evaluation. The SC2/InflA/InflB-UCT allows for efficient high-throughput testing for all three pathogens, providing streamlined diagnostics and resource conservation during the influenza season.

JOURNAL OF MEDICAL MICROBIOLOGY (2021)

Article Virology

A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades

Mohammad Tanvir Islam et al.

Summary: The study introduces a rapid, simple, and cost-effective multiplex PCR assay to identify six phylogenetic clades of SARS-CoV-2, which has been optimized and validated. This multiplex detection method can quickly and inexpensively discriminate the phylogenetic clade variants of infected individuals.

JOURNAL OF MEDICAL VIROLOGY (2021)

Article Environmental Sciences

SARS-CoV-2 sewage surveillance in low-income countries: potential and challenges

Juliana Calabria de Araujo et al.

Summary: This paper reviews recent findings on the detection of SARS-CoV-2 in sewage samples and discusses the potential of wastewater-based surveillance (WBS) as a complementary tool in the fight against COVID-19 spread. Challenges in estimating the number of infected people in a community using different protocols and the importance of tailored monitoring and sampling plans are highlighted. WBS has the potential to play a crucial and cost-effective role in monitoring virus circulation and assessing the real prevalence of COVID-19 in areas with low sanitation levels like urban agglomerates in Brazil.

JOURNAL OF WATER AND HEALTH (2021)

Article Environmental Sciences

SARS-CoV-2 has been circulating in northern Italy since December 2019: Evidence from environmental monitoring

Giuseppina La Rosa et al.

Summary: Italy had SARS-CoV-2 circulating as early as the end of 2019, with the virus spreading in different geographic regions simultaneously. The study emphasizes the importance of environmental surveillance as an early warning system to monitor virus levels and identify outbreaks before they are officially reported to the healthcare system.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Immunology

Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system

Hsing-Yi Chung et al.

Summary: This study evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, and developed a rapid total nucleic acid extraction method for simultaneous detection of common cold viral pathogens. The BD MAX Platform demonstrated high sensitivity and accuracy for detecting common cold viral pathogens.

EMERGING MICROBES & INFECTIONS (2021)

Review Environmental Sciences

Wastewater surveillance for SARS-CoV-2: Lessons learnt from recent studies to define future applications

Mohamed Hamouda et al.

Summary: Wastewater-based epidemiology has been successful in detecting the spread of SARS-CoV-2, especially in situations where clinical testing is limited and infected individuals may not show symptoms for a long period. The use of wastewater surveillance has shown the impact of lockdown measures on virus spread, leading to the development of a specialized framework for monitoring SARS-CoV-2. Despite challenges, future research should consider various factors such as safety, efficacy, and standardized protocols for large-scale international studies.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Environmental Sciences

SARS-CoV-2 RNA monitoring in wastewater as a potential early warning system for COVID-19 transmission in the community: A temporal case study

Warish Ahmed et al.

Summary: Detection of SARS-CoV-2 RNA in wastewater can serve as an early warning system to identify viral hotspots and target localized public health responses. This method provides unique information on asymptomatic and pre-symptomatic cases that may not be captured by clinical testing, showing potential for effective disease surveillance.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Environmental Sciences

Detection of SARS-CoV-2 RNA in hospital wastewater from a low COVID-19 disease prevalence area

Jose Goncalves et al.

Summary: Previous studies have reported the presence of viral RNA in stool samples from individuals with SARS-CoV and MERS-CoV, suggesting that wastewater from affected communities may contain SARS-CoV-2 RNA. Recent studies have also found SARS-CoV-2 RNA in human feces. Wastewater-based epidemiology is a promising approach to monitor the prevalence of COVID-19 in communities, as demonstrated by the first detection of SARS-CoV-2 RNA in untreated wastewater in Slovenia.

SCIENCE OF THE TOTAL ENVIRONMENT (2021)

Article Engineering, Environmental

Surveillance of wastewater revealed peaks of SARS-CoV-2 preceding those of hospitalized patients with COVID-19

Fredy Saguti et al.

Summary: The study revealed that SARS-CoV-2 can be detected in wastewater, and its levels reflect the extent of transmission in society. Viral peaks occurred approximately every four weeks, preceding fluctuations in newly hospitalized patients by 19-21 days. Local differences in viral spread were observed, with varying amounts of virus found in wastewater from different areas of the same city, possibly reflecting the transmission patterns of infected individuals.

WATER RESEARCH (2021)

Article Biochemistry & Molecular Biology

Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2

Chantal B. F. Vogels et al.

Summary: With the emergence of SARS-CoV-2 variants with increased transmissibility and immune escape, targeted surveillance is urgently needed. The B.1.1.7 variant can be detected by a specific deletion in the spike gene, but other variants like B.1.351 and P.1 cannot. An open-source PCR assay targeting a deletion in the ORF1a gene has been designed and validated to enhance surveillance for these variants.

PLOS BIOLOGY (2021)

Article Infectious Diseases

Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR

Remco de Kock et al.

Summary: The study developed a one-step droplet digital RT-PCR multiplex assay for reliable quantification of SARS-CoV-2 RNA, which could be used for screening and monitoring Covid-19 patients. By determining the fractional abundance of viral RNA with respect to a human housekeeping gene, viral loads from different samples can be compared.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2021)

Article Infectious Diseases

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman et al.

EUROSURVEILLANCE (2020)

Article Biochemistry & Molecular Biology

The Architecture of SARS-CoV-2 Transcriptome

Dongwan Kim et al.

Letter Engineering, Environmental

Letter to the Editor: Wastewater-Based Epidemiology Can Overcome Representativeness and Stigma Issues Related to COVID-19

Michio Murakami et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2020)

Article Environmental Sciences

Aerosol transmission of SARS-CoV-2? Evidence, prevention and control

Song Tang et al.

ENVIRONMENT INTERNATIONAL (2020)

Editorial Material Construction & Building Technology

Recognizing and controlling airborne transmission of SARS-CoV-2 in indoor environments

Joseph G. Allen et al.

INDOOR AIR (2020)

Article Microbiology

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets

Chantal B. F. Vogels et al.

NATURE MICROBIOLOGY (2020)

Article Virology

Clinical evaluation of AusDiagnostics SARS-CoV-2 multiplex tandem PCR assay

Lucy O Attwood et al.

JOURNAL OF CLINICAL VIROLOGY (2020)

Article Virology

Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR

Garrett A. Perchetti et al.

JOURNAL OF CLINICAL VIROLOGY (2020)

Review Engineering, Environmental

Coronavirus in water environments: Occurrence, persistence and concentration methods - A scoping review

Giuseppina La Rosa et al.

WATER RESEARCH (2020)

Article Engineering, Environmental

SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area

Walter Randazzo et al.

WATER RESEARCH (2020)

Article Environmental Sciences

First detection of SARS-CoV-2 in untreated wastewaters in Italy

Giuseppina La Rosa et al.

SCIENCE OF THE TOTAL ENVIRONMENT (2020)

Article Biochemistry & Molecular Biology

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR

Eriko Kudo et al.

PLOS BIOLOGY (2020)

Review Environmental Sciences

Wastewater-Based Epidemiology (WBE) and Viral Detection in Polluted Surface Water: A Valuable Tool for COVID-19 Surveillance-A Brief Review

Maria de Lourdes Aguiar-Oliveira et al.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH (2020)

Article Biochemistry & Molecular Biology

A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection

Martin A. M. Reijns et al.

PLOS BIOLOGY (2020)

Review Environmental Sciences

SARS-CoV-2 from faeces to wastewater treatment: What do we know. A review

Paola Foladori et al.

SCIENCE OF THE TOTAL ENVIRONMENT (2020)

Article Environmental Sciences

SARS-CoV-2 in river water: Implications in low sanitation countries

Laura Guerrero-Latorre et al.

SCIENCE OF THE TOTAL ENVIRONMENT (2020)

Article Environmental Sciences

First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA

Samendra P. Sherchan et al.

SCIENCE OF THE TOTAL ENVIRONMENT (2020)

Article Multidisciplinary Sciences

Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR

Michela Deiana et al.

SCIENTIFIC REPORTS (2020)

Letter Immunology

Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Jesse J. Waggoner et al.

EMERGING INFECTIOUS DISEASES (2020)

Article Immunology

Environmental Surveillance for Polioviruses in the Global Polio Eradication Initiative

Humayun Asghar et al.

JOURNAL OF INFECTIOUS DISEASES (2014)