Journal
RNA BIOLOGY
Volume 18, Issue -, Pages 747-756Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2021.1989218
Keywords
MicroRNA biomarker; cancer detection; blood-based microRNA; clinical bioinformatics; TCGA
Categories
Funding
- National cancer control charity fundation
- Tehran University of Medical Sciences and Health Services
- Royan Institute
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This study aimed to assess the relationship between blood-based miRNAs and demographic characteristics of breast cancer patients, finding a potential multi-marker panel for early detection of breast cancer with acceptable sensitivity, specificity, and accuracy. The panel also showed promise in distinguishing benign breast diseases from breast cancer patients.
Breast cancer (BC) as a leading cause of cancer death among women, exhibits a wide range of genetic heterogeneity in affected individuals. Satisfactory management of BC depends on early diagnosis and proper monitoring of patients' response to therapy. In this study, we aimed to assess the relation between the expression patterns of blood-based microRNAs (miRNAs) with demographic characteristics of the patients with BC in an attempt to find novel diagnostic markers for BC with acceptable precision in clinical applications. To this end, we performed comprehensive statistical analysis of the data of the Cancer Genome Atlas (TCGA) database and the blood miRNome dataset (GSE31309). As a result, 21 miRNAs were selected for experimental verification by quantitative RT-PCR on blood samples of 70 BC patients and 60 normal individuals (without any lesions or benign breast diseases). Statistical one-way ANOVA revealed no significant difference in the blood levels of the selected miRNAs in BC patients compared to any lesions or benign breast diseases. However, the multi-marker panel consisting of hsa-miR-106b-5p, -126-3p, -140-3p, -193a-5p, and -10b-5p could detect early-stages of BC with 0.79 sensitivity, 0.86 specificity and 0.82 accuracy. Furthermore, this multi-marker panel showed the potential of detecting benign breast diseases from BC patients with 0.67 sensitivity, 0.80 specificity, and 0.74 accuracy. In conclusion, these data indicate that the present panel might be considered an asset in detecting benign breast disease and BC.
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