Journal
INSECT MOLECULAR BIOLOGY
Volume 25, Issue 2, Pages 171-180Publisher
WILEY
DOI: 10.1111/imb.12211
Keywords
Apis mellifera; induction; P450; esterase; thiacloprid; metabolism
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Funding
- AHDB Cereals and Oilseeds, a division of the Agriculture and Horticulture Development Board, UK
- Biotechnology and Biological Sciences Research Council of the UK
- BBSRC [BBS/E/C/00005193] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005193] Funding Source: researchfish
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Honey bees, Apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme(s) involved remain to be characterized. In this work, a pretreatment of honey bees with a sublethal dose of thiacloprid resulted in induced insensitivity to the same compound immediately following thiacloprid feeding. A longer pretreatment time resulted in no, or increased, sensitivity. Transcriptome profiling, using microarrays, identified a number of genes encoding detoxification enzymes that were over-expressed significantly in insecticide-treated bees compared with untreated controls. These included five P450s, CYP6BE1, CYP305D1, CYP6AS5, CYP315A1, CYP301A1, and a carboxyl/cholinesterase (CCE) CCE8. Four of these P450s were functionally expressed in Escherichia coli and their ability to metabolize thiacloprid examined by liquid chromatography-mass spectrometry (LC-MS) analysis.
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