Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 76, Issue -, Pages 109-117Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2016.07.008
Keywords
ABC transporter; Bt Cry1A toxin; Cadherin; Heliothis virescens; Insecticide resistance
Categories
Funding
- Max-Planck-Gesellschaft
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Insecticidal crystal (Cry) proteins from Bacillus thuringiensis (Bt) are highly active against Lepidoptera. However, field-evolved resistance to Bt toxins is on the rise. The 12-cadherin domain protein HevCaLP and the ABC transporter HevABCC2 are both genetically linked to Cry toxin resistance in Heliothis virescens. We investigated their interaction using stably expressing non-lytic clonal Sf9 cell lines expressing either protein or both together. Untransfected Sf9 cells are innately sensitive to CrylCa toxin, but not to CrylA toxins; and quantitative PCR revealed negligible expression of genes involved in CrylA toxicity such as cadherin, ABCC2, alkaline phosphatase (ALP) and aminopeptidase N (APN). CrylAa, CrylAb or CrylAc caused swelling of Sf9 cells expressing HevABCC2, and caused faster swelling, lysis and up to 86% mortality in cells expressing both proteins. No such effect was observed in control Sf9 cells or in cells expressing only HevCaLP. The results of a mixing experiment demonstrated that both proteins need to be expressed within the same cell for high cytotoxicity, and suggest a novel role for HevCaLP. Binding assays showed that the toxin-receptor interaction is specific. Our findings confirm that HevABCC2 is the central target in CrylA toxin mode of action, and that HevCaLP plays a supporting role in increasing CrylA toxicity. (C) 2016 Elsevier Ltd. All rights reserved.
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