4.4 Article

Transgenic expression of rice OsPHR2 increases phosphorus uptake and yield in wheat

Journal

PROTOPLASMA
Volume 259, Issue 5, Pages 1271-1282

Publisher

SPRINGER WIEN
DOI: 10.1007/s00709-021-01702-5

Keywords

Transgenic wheat; OsPHR2; Phosphorus uptake; Grain yield

Funding

  1. National Natural Science Foundation of China [31701510]
  2. Genetically Modified Organisms Breeding Major Projects of China [2016ZX08002005-004]
  3. Science and Technology project of Henan Province [192102110015]
  4. Science Technology Foundation for Outstanding Young Scientists of Henan Academy of Agricultural Sciences [2020YQ29]

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Introduction of OsPHR2 gene into wheat can enhance P uptake and utilization, with more prominent effect under low P conditions.
Oryza sativa PHOSPHATE RESPONSE2 (OsPHR2) can promote the uptake and use of phosphorus (P) in rice. We introduced OsPHR2 into the winter wheat (Triticum aestivum L.) variety Zhengmai0856. OsPHR2 was integrated into the wheat genome with two copy numbers and could be correctly transcribed and expressed. OsPHR2 was mainly expressed in the leaves at the seedling stage. From the jointing to filling stage, OsPHR2 was mainly expressed in the roots, followed by the leaves, with a low expression level in detected the tassels and stems. The transgenic lines exhibited higher P accumulation at each growth stage and increased P uptake intensity in comparison to the wild type under low P and high P conditions. Analysis of the root characteristics showed that the transgenic expression of OsPHR2 increased the maximum root length, total root length, root-to-shoot ratio, and root volume under the conditions of P deficiency or low P. A field experiment showed that the transgenic lines had a higher grain yield than the wild type under low P and high P conditions. The yield of the transgenic lines increased by 6.29% and 3.73% on average compared with the wild type under low P and high P conditions, respectively. Thus, the transgenic expression of OsPHR2 could increase P uptake and yield in wheat, but the effect was more prominent under low P conditions.

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