Journal
PROTEIN SCIENCE
Volume 31, Issue 3, Pages 639-651Publisher
WILEY
DOI: 10.1002/pro.4259
Keywords
cell-free expression; complexes; eukaryotic membrane proteins; high throughput; lipidic mimetics
Categories
Funding
- NIH NIGMS through the Center on Membrane Protein Production and Analysis [5P41GM116799]
- U.S. Department of Energy, Office of Science, Biological and Environmental Research, as part of the Quantitative Plant Science Initiative at BNL
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Membrane proteins play important roles in cellular function and metabolism, but their study is often hindered by difficulties in expression and purification. This study presents a high-throughput screening workflow for eukaryotic membrane protein targets using cell-free protein synthesis in various lipidic mimetics, showing that 57% of the tested membrane proteins can be expressed in soluble form. The results demonstrate the utility of using cell-free systems for screening and production of eukaryotic membrane proteins.
Membrane proteins play essential roles in cellular function and metabolism. Nonetheless, biophysical and structural studies of membrane proteins are impeded by the difficulty of their expression in and purification from heterologous cell-based systems. As an alternative to these cell-based systems, cell-free protein synthesis has proven to be an exquisite method for screening membrane protein targets in a variety of lipidic mimetics. Here we report a high-throughput screening workflow and apply it to screen 61 eukaryotic membrane protein targets. For each target, we tested its expression in lipidic mimetics: two detergents, two liposomes, and two nanodiscs. We show that 35 membrane proteins (57%) can be expressed in a soluble fraction in at least one of the mimetics with the two detergents performing significantly better than nanodiscs and liposomes, in that order. Using the established cell-free workflow, we studied the production and biophysical assays for mitochondrial pyruvate carrier (MPC) complexes. Our studies show that the complexes produced in cell-free are functionally competent in complex formation and substrate binding. Our results highlight the utility of using cell-free systems for screening and production of eukaryotic membrane proteins.
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