Peptides as 3D printable feedstocks: Design strategies and emerging applications

Peptide hydrogels have proven to be a critical building block for a range of scientific applications due to their high water capacity, tunable mechanical properties, and modular synthetic design. Particular interest stems from the biomedical realm due to their inherent biomimetic structure and biocompatibility, which has prompted their use as 3D printable matrices. In this review, we will discuss the two main strategies for preparing peptide hydrogels using solid-phase peptide synthesis (SPPS) and N-carboxyanhydride ring opening polymerization (NCA ROP) to construct the linear chains followed by gelation through either non-covalent interactions or covalent crosslinking using light or enzymes as a trigger. These synthetic approaches are summarized with a view to the applicability of hydrogel building blocks in 3D printing and representative examples illustrating the future outlook and possible research directions for the field.(c) 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )

Automated summary

Peptide hydrogels are critical building blocks for various scientific applications due to their high water capacity, tunable mechanical properties, and modular synthetic design. They are particularly interesting in the biomedical field due to their biomimetic structure and biocompatibility, making them suitable for 3D printing matrices. This review discusses two strategies for preparing peptide hydrogels using solid-phase peptide synthesis and N-carboxyanhydride ring opening polymerization, and explores their applicability in 3D printing and potential research directions.