Journal
PROCESS BIOCHEMISTRY
Volume 110, Issue -, Pages 251-262Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2021.08.006
Keywords
Trace metals; Chinese hamster ovary cells; Recombinant proteins; Product quality; Glycosylation; Medium formulation; Process variability
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The availability of trace metals can impact cell culture performance, productivity, and product quality, as well as the stability of other trace metals and components in the culture medium. Understanding the role and impact of trace metals is crucial for developing strategies to achieve targeted process parameters and increase process robustness.
Replacement of serum and increasing use of chemically defined media demands optimisation of trace metal components for biomanufacturing applications. Trace metal availability can impact culture performance, productivity and product quality. Several trace metals are cofactors of metabolic and other enzymes, and thus their availability regulates cellular metabolism. Additionally, they can also affect the availability of other trace metals and stability of some medium components. Such factors also need to be considered while formulating trace metal concentrations in the culture medium. Due to their very low concentrations, these components are susceptible to substantial variability arising from contaminants from other raw material and leaching from process equipment and can contribute to process variability. Understanding the role and impact of trace metals will help develop strategies to achieve targeted process parameters and increase process robustness vis-`a-vis any lot-to-lot variability in trace metal concentration in culture medium. This review describes the role of trace metals, particularly manganese, copper and zinc, in central carbon metabolism to aid in understanding the basis of metal-mediated effects on culture performance and provides a comprehensive review of the reported impact of trace metals on CHO cell culture performance and recombinant protein quality.
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