Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 119, Issue 3, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2103527119
Keywords
effector; small RNA; symbiosis; mycorrhizae; plant defense
Categories
Funding
- Western Sydney University
- Hawkesbury Foundation
- Australian Research Council [DE150100408]
- Laboratory of Excellence ARBRE [ANR-11LABX-0002-01]
- Office of Science of the US DOE [DE-AC02-05CH11231]
- Australian Research Council [DE150100408] Funding Source: Australian Research Council
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This study discovered a novel fungal miRNA that is transferred during the mutualistic interaction between ectomycorrhizal fungi and Eucalyptus grandis, indicating the crucial role of miRNAs in the developmental process of mutualistic symbioses.
Small RNAs (sRNAs) are known to regulate pathogenic plant- microbe interactions. Emerging evidence from the study of these model systems suggests that microRNAs (miRNAs) can be translocated between microbes and plants to facilitate symbiosis. The roles of sRNAs in mutualistic mycorrhizal fungal interactions, however, are largely unknown. In this study, we characterized miRNAs encoded by the ectomycorrhizal fungus Pisolithus microcarpus and investigated their expression during mutualistic interaction with Eucalyptus grandis. Using sRNA sequencing data and in situ miRNA detection, a novel fungal miRNA, Pmic_miR-8, was found to be transported into E. grandis roots after interaction with P. microcarpus. Further characterization experiments demonstrate that inhibition of Pmic_miR-8 negatively impacts the maintenance of mycorrhizal roots in E. grandis, while supplementation of Pmic_ miR-8 led to deeper integration of the fungus into plant tissues. Target prediction and experimental testing suggest that Pmic_ miR-8 may target the host NB-ARC domain containing transcripts, suggesting a potential role for this miRNA in subverting host signaling to stabilize the symbiotic interaction. Altogether, we provide evidence of previously undescribed cross-kingdom sRNA transfer from ectomycorrhizal fungi to plant roots, shedding light onto the involvement of miRNAs during the developmental process of mutualistic symbioses.
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