Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 119, Issue 1, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2116765118
Keywords
photosynthesis; photosystem II; water oxidation; oxygen-evolving complex; PsbQ
Categories
Funding
- Department of Energy, Office of Basic Energy Sciences [DE-FG02-05ER15646, DE-SC0005291, DE-SC0001423]
- Department of Energy, Division of Chemical Sciences [DE-FG02-05ER15646, DE-SC0005291, DE-SC0001423]
- National Institute of General Medical Sciences of the NIH [K99GM140174]
- Office of the Dean at the Yale School of Medicine
- Office of the Provost at Yale University
- U.S. Department of Energy (DOE) [DE-SC0005291, DE-FG02-05ER15646, DE-SC0001423] Funding Source: U.S. Department of Energy (DOE)
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The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
Photosystem II (PSII) enables global-scale, light-driven water oxidation. Genetic manipulation of PSII from the mesophilic cyanobacterium Synechocystis sp. PCC 6803 has provided insights into the mechanism of water oxidation; however, the lack of a high-resolution structure of oxygen-evolving PSII from this organism has limited the interpretation of biophysical data to models based on structures of thermophilic cyanobacterial PSII. Here, we report the cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 at 1.93-angstrom resolution. A number of differences are observed relative to thermophilic PSII structures, including the following: the extrinsic subunit PsbQ is maintained, the C terminus of the D1 subunit is flexible, some waters near the active site are partially occupied, and differences in the PsbV subunit block the Large (O1) water channel. These features strongly influence the structural picture of PSII, especially as it pertains to the mechanism of water oxidation.
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