Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 51, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2112561118
Keywords
retina; microglia; capillary regulation; fractalkine; diabetes
Categories
Funding
- National Health and Medical Research Council [APP-2000669, APP-1138509]
- Australian Research Council [DP160102642, DP200102001, FT130100338]
- Australian Research Council [DP200102001, FT130100338] Funding Source: Australian Research Council
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Local blood flow control within the central nervous system (CNS) is dependent on coordination between neurons, glia, and blood vessels. This study explored the role of microglia in retinal vasoregulation, showing that microglia actively participate in the neurovascular unit and contribute to vascular compromise during diabetic retinopathy. The findings suggest that aberrant microglial-vascular function may play a role in early vascular changes in diabetic retinopathy.
Local blood flow control within the central nervous system (CNS) is critical to proper function and is dependent on coordination between neurons, glia, and blood vessels. Macroglia, such as astrocytes and Muller cells, contribute to this neurovascular unit within the brain and retina, respectively. This study explored the role of microglia, the innate immune cell of the CNS, in retinal vasoregulation, and highlights changes during early diabetes. Structurally, microglia were found to contact retinal capillaries and neuronal synapses. In the brain and retinal explants, the addition of fractalkine, the sole ligand for monocyte receptor Cx3cr1, resulted in capillary constriction at regions of microglial contact. This vascular regulation was dependent on microglial Cx3cr1 involvement, since genetic and pharmacological inhibition of Cx3cr1 abolished fractalkine-induced constriction. Analysis of the microglial transcriptome identified several vasoactive genes, including angiotensinogen, a constituent of the renin-angiotensin system (RAS). Subsequent functional analysis showed that RAS blockade via candesartan abolished microglial-induced capillary constriction. Microglial regulation was explored in a rat streptozotocin (STZ) model of diabetic retinopathy. Retinal blood flow was reduced after 4 wk due to reduced capillary diameter and this was coincident with increased microglial association. Functional assessment showed loss of microglial-capillary response in STZ-treated animals and transcriptome analysis showed evidence of RAS pathway dysregulation in microglia. While candesartan treatment reversed capillary constriction in STZ-treated animals, blood flow remained decreased likely due to dilation of larger vessels. This work shows microglia actively participate in the neurovascular unit, with aberrant microglial-vascular function possibly contributing to the early vascular compromise during diabetic retinopathy.
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