Journal
PLOS ONE
Volume 16, Issue 12, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0260010
Keywords
-
Categories
Funding
- Office of The Director, National Institutes of Health [P51OD011106]
- WNPRC Pilot Grant program
- NIH [R01 AI08415]
- National Cancer Institute, National Institutes of Health [75N91019D00024]
- Australian National Health and Medical Research Council (NHMRC) [1080001, 1052979, 1067590]
- National Health and Medical Research Council of Australia [1067590] Funding Source: NHMRC
Ask authors/readers for more resources
Little is known about how specific individual viral lineages persist during acute HIV/SIV infection into chronic infection. This study used molecularly barcoded SIV to track distinct viral lineages in macaques and found that the challenge route may restrict the transmission of individual viral lineages. Additionally, the emergence of escape variants in acutely targeted epitopes can occur on multiple virus templates simultaneously, but elimination of some templates may be due to additional host factors.
Little is known about how specific individual viral lineages replicating systemically during acute Human Immunodeficiency Virus or Simian Immunodeficiency Virus (HIV/SIV) infection persist into chronic infection. In this study, we use molecularly barcoded SIV (SIVmac239M) to track distinct viral lineages for 12 weeks after intravenous (IV) or intrarectal (IR) challenge in macaques. Two Mafa-A1*063+ cynomolgus macaques (Macaca fascicularis, CM) were challenged IV, and two Mamu-A1*001+ rhesus macaques (Macaca mulatta, RM) were challenged IR with 200,000 Infectious Units (IU) of SIVmac239M. We sequenced the molecular barcode of SIVmac239M from all animals over the 12 weeks of the study to characterize the diversity and persistence of virus lineages. During the first three weeks post-infection, we found ~70-560 times more unique viral lineages circulating in the animals challenged IV compared to those challenged IR, which is consistent with the hypothesis that the challenge route is the primary driver restricting the transmission of individual viral lineages. We also characterized the sequences of T cell epitopes targeted during acute SIV infection, and found that the emergence of escape variants in acutely targeted epitopes can occur on multiple virus templates simultaneously, but that elimination of some of these templates is likely a consequence of additional host factors. These data imply that virus lineages present during acute infection can still be eliminated from the systemic virus population even after initial selection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available