Identification of a wild carrot as carrot psylla (Bactericera trigonica) attractant and host plant chemistry

Carrot psylla is one of the devastating pests of carrot throughout northern Europe and the Mediterranean basin. Here we characterized the behavioral response of psylla females towards different carrot germplasm and identified the chemical cues involved in the host selection of psylla females by oviposition choice experiments and metabolic profiling of leaf volatiles. In choice assays, carrot psylla displayed differential responses to tested 14 germplasm. Among germplasm, wild accessions 21793 and 20465 were highly preferred by carrot psylla, while wild accessions 20465 and the orange cultivar Nairobi were less. In non-choice experiments conducted only with this four-germplasm revealed that the carrot psylla females gave higher preference to the Nairobi and wild accession 20465, indicating the vicinity to other host plants in the same area might affect female preference. Moreover, the nymph development and survival experiments showed the lowest nymphs survival rate on the wild accessions 21793 and 20497. Furthermore, the volatile emissions among different carrot cultivars infested with psylla showed qualitative and quantitative differences versus intact plants. Among these volatiles, apiol, fl-asarone, myristicin, and sabinene showed a relationship with psyllas growth and survival. We also showed that myristicin and sabinene exogenous applications caused a dramatic reduction in the number of eggs laid by psylla and subsequent nymph survival. This is an initial study of the volatiles that mediate attraction and oviposition preference of carrot psylla in response to its host plant. The results from this study provide baseline information for the development of new control strategies against carrot psylla.

Automated summary

Carrot psylla females exhibited differential preferences towards different carrot germplasm, with wild accessions 21793 and 20465 being highly preferred. Volatile emissions from infested carrot cultivars differed qualitatively and quantitatively compared to intact plants, with certain volatiles like apiol, fl-asarone, myristicin, and sabinene showing associations with psylla growth and survival. Exogenous application of myristicin and sabinene led to a significant reduction in psylla oviposition and nymph survival. This study provides valuable information for developing new control strategies against carrot psylla.