Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 171, Issue -, Pages 105-114Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2021.12.031
Keywords
Rice; Proteomics; Bacterial blight disease; Arginase; Low-abundance proteins
Categories
Funding
- National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea [PJ01570601]
- National Research Foundation of Korea (NRF) [2019R1A2C2085868, 2021R1A4A2001968]
- National Research Foundation of Korea [2021R1A4A2001968, 2019R1A2C2085868] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study investigated the proteome profiles of rice cultivars in response to Xanthomonas oryzae pv. oryzae (Xoo) infection, identifying key proteins involved in plant defense response. Among them, mitochondrial arginase-1 (OsARG1) showed significant accumulation and function in the resistant rice cultivar, enhancing tolerance against Xoo when overexpressed in susceptible cultivars. The results highlight the role of OsARG1 in plant defense against Xoo.
Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is one of the major threats to rice productivity. Yet, the molecular mechanism of rice-Xoo interaction is elusive. Here, we report comparative proteome profiles of Xoo susceptible (Dongjin) and resistant (Hwayeong) cultivars of rice in response to two-time points (3 and 6 days) of Xoo infection. Low-abundance proteins were enriched using a protamine sulfate (PS) precipitation method and isolated proteins were quantified by a label-free quantitative analysis, leading to the identification of 3846 proteins. Of these, 1128 proteins were significantly changed between mock and Xoo infected plants of Dongjin and Hwayeong cultivars. Based on the abundance pattern and functions of the identified proteins, a total of 23 candidate proteins were shortlisted that potentially participate in plant defense against Xoo in the resistant cultivar. Of these candidate proteins, a mitochondrial arginase-1 showed Hwayeong specific abundance and was significantly accumulated following Xoo inoculation. Overexpression of arginase 1 (OsArg 1) in susceptible rice cultivar (Dongjin) resulted in enhanced tolerance against Xoo as compared to the wild-type. In addition, expression analysis of defense-related genes encoding PR1, glucanase I, and chitinase II by qRT-PCR showed their enhanced expression in the overexpression lines as compared to wild-type. Taken together, our results uncover the proteome changes in the rice cultivars and highlight the functions of OsARG1 in plant defense against Xoo.
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