Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 170, Issue -, Pages 234-248Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2021.12.009
Keywords
Comparative proteomics; Salt ions; Sesuvium portulacastrum; Sodium chloride; True halophyte
Categories
Funding
- Key Science and Technology Program of Hainan Province [ZDKJ202008]
- Natural Science Foundation of Hainan Province [2019RC200]
- National Natural Science Foundation of China [31860224]
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This study conducted comparative physiological and proteomic analyses to identify the different responses of Sesuvium portulacastrum leaves to various salt ions. Fifty-three differentially accumulated proteins were identified, with different expression patterns depending on the salt ions. These proteins were involved in photosynthesis, carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Enzyme activity analysis revealed the induction of P-ATPase and V-ATPase by certain salt ions, while V-PPase activity was significantly increased by KNO3 but inhibited by NaCl.
Sesuvium portulacastrum is a true halophyte and shows an optimal development under moderate salinity with large amounts of salt ions in its leaves. However, the specific proteins in response to salt ions are remained unknown. In this study, comparative physiological and proteomic analyses of different leaves subject to NaCl, KCl, NaNO3 and KNO3 were performed. Chlorophyll content was decreased under the above four kinds of salt treatments. Starch and soluble sugar contents changed differently under different salt treatments. A total of 53 differentially accumulated proteins (DAPs) were identified by mass spectrometry. Among them, 13, 25, 26 and 25 DAPs were identified after exposure to KCl, NaCl, KNO3, and NaNO3, respectively. These DAPs belong to 47 unique genes, and 37 of them are involved in protein-protein interactions. These DAPs displayed different expression patterns after treating with different salt ions. Functional annotation revealed they are mainly involved in photosynthesis, carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of sec-ondary metabolites. Genes and proteins showed different expression profiles under different salt treatments. Enzyme activity analysis indicated P-ATPase was induced by KCl, NaCl and NaNO3, V-ATPase was induced by KCl and NaCl, whereas V-PPase activity was significantly increased after application of KNO3, but sharply inhibited by NaCl. These results might deepen our understanding of responsive mechanisms in the leaves of S. portulacastrum upon different salt ions.
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