Journal
PLANT PHYSIOLOGY
Volume 188, Issue 1, Pages 97-110Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/plphys/kiab504
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Funding
- Chinese Scholarship Council (CSC)
- Peterson scholarship
- European Research Council (ERC) [647148]
- Deutsche Forschungsgemeinschaft [DFG] [GR_2104/4-1]
- DFG [GR4559_4-1, GR4559_5-1, EXC-2048/1, 390686111]
- Alexander von Humboldt-Stiftung [3.5-JPN 1164674-HFST-P]
- Japan Society for the Promotion of Science [JSPS Overseas Research Fellowships] [201960008]
- Nikon Imaging Center (NIC) of Heidelberg University
- European Research Council (ERC) [647148] Funding Source: European Research Council (ERC)
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The development of a genetically-encoded ratiometric SL signaling sensor allows for rapid response to changes in SL levels in plant cells, with different cell types showing varying dynamics in response to SL levels. It indicates that SL signaling in plants exhibits specificity in distribution among different cell types.
Strigolactones (SLs) are a class of plant hormones that mediate biotic interactions and modulate developmental programs in response to endogenous and exogenous stimuli. However, a comprehensive view on the spatio-temporal pattern of SL signaling has not been established, and tools for a systematic in planta analysis do not exist. Here, we present Strigo-D2, a genetically encoded ratiometric SL signaling sensor that enables the examination of SL signaling distribution at cellular resolution and is capable of rapid response to altered SL levels in intact Arabidopsis (Arabidopsis thaliana) plants. By monitoring the abundance of a truncated and fluorescently labeled SUPPRESSOR OF MAX2 1-LIKE 6 (SMXL6) protein, a proteolytic target of the SL signaling machinery, we show that all cell types investigated have the capacity to respond to changes in SL levels but with very different dynamics. In particular, SL signaling is pronounced in vascular cells but low in guard cells and the meristematic region of the root. We also show that other hormones leave Strigo-D2 activity unchanged, indicating that initial SL signaling steps work in isolation from other hormonal signaling pathways. The specificity and spatio-temporal resolution of Strigo-D2 underline the value of the sensor for monitoring SL signaling in a broad range of biological contexts with highly instructive analytical depth.
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