Transcriptome Changes Induced by Botrytis cinerea Stress and Weighted Gene Co-expression Network Analysis (WGCNA) in Actinidia chinensis

Kiwifruit (Actinidia chinensis) is rich in dietary fiber and vitamin C and has high commercial value. However, it is susceptible to Botrytis cinerea pathogenesis. This fungus causes gray mold rot which negatively impacts kiwifruit quality. It also induces changes in superoxide dismutase, peroxidase, and catalase activity. Peroxidase and superoxide dismutase activities were detected at 1 dpi, increased till 4 dpi, and decreased by 5 dpi. Catalase was activated at 2 dpi, increased thereafter, and declined by 5 dpi. RNA-seq identified 2726 unique differentially expressed genes. The fold change was at least two, and the false discovery rate was less than 0.01. The 233 genes relevant to B. cinerea response were modulated in the first three infection stages. A weighted gene co-expression network analysis identified genes and modules in the transcriptome datasets that were responsive to B. cinerea. A network analysis disclosed the families Plant-pathogen interactions, Lipid metabolism, Phytohormone signaling, Transcription factor, Cell wall biogenesis and Phenylpropanoid biosynthesis possibly regulating B. cinerea response. The hub genes identified here are potential targets for breeding kiwifruit with improved biotic stress tolerance. This work lays an empirical foundation for investigating the proteins in kiwifruit that are modulated in response to B. cinerea pathogenesis.

Automated summary

Kiwifruit is rich in dietary fiber and vitamin C, but it is susceptible to Botrytis cinerea pathogenesis. This study found that the fungus caused changes in enzyme activity and identified thousands of differentially expressed genes. Certain gene families and metabolic pathways were also found to be involved in the response to B. cinerea. The hub genes identified in this study could be potential targets for breeding kiwifruit with improved resistance to biotic stress.