Journal
PLANT JOURNAL
Volume 109, Issue 4, Pages 831-843Publisher
WILEY
DOI: 10.1111/tpj.15596
Keywords
HISTONE DEACETYLASE 6; MULTICOPY SUPRESSOR OF IRA1; flowering; Arabidopsis thaliana; FLOWERING LOCUS C; MADS AFFECTING FLOWERING 4; MADS AFFECTING FLOWERING 5
Categories
Funding
- Laboratory of Lingnan Modern Agriculture Project [NT2021004]
- Special Fund for Scientific Innovation Strategy- Construction of High Level from the Guangdong Academy of Agriculture Science [R2021YJ-QG001]
- Open Research Fund of the Guangdong Key Laboratory for New Technology Research of Vegetables [202001]
- Science and Technology Planning Project of Guangzhou [202102021008]
- Foundation Project of the President of Guangdong Academy of Agricultural Sciences [202021]
- Guangdong Key Lab for Crop Germplasm Resources Preservation and Utilization [2020B121201008]
- Ministry of Science and Technology of Taiwan [108-2311-B-002-013-MY3, 110-2311-B-002-027]
- National Taiwan University [NTU-CC-110L893601]
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The study demonstrates that MSI1 and HDA6 interact to repress flowering time control genes through histone modifications. HDA6-mediated H3K9 deacetylation is dependent on MSI1, while H3K27Me3 mediated by PRC2 containing MSI1 is also dependent on HDA6. The findings reveal the crucial role of the HDA6-MSI1 module in mediating the interaction between histone deacetylation and H3K27Me3 to repress gene expression.
MULTICOPY SUPPRESSOR OF IRA1 (MSI1) is a conserved subunit of Polycomb Repressive Complex 2 (PRC2), which mediates gene silencing by histone H3 lysine 27 trimethylation (H3K27Me3). Here, we demonstrated that MSI1 interacts with the RPD3-like histone deacetylase HDA6 both in vitro and in vivo. MSI1 and HDA6 are involved in flowering and repress the expression of FLC, MAF4, and MAF5 by removing H3K9 acetylation but adding H3K27Me3. Chromatin immunoprecipitation analysis showed that HDA6 and MSI1 interdependently bind to the chromatin of FLC, MAF4, and MAF5. Furthermore, H3K9 deacetylation mediated by HDA6 is dependent on MSI1, while H3K27Me3 mediated by PRC2 containing MSI1 is also dependent on HDA6. Taken together, these data indicate that MSI1 and HDA6 act interdependently to repress the expression of FLC, MAF4, and MAF5 through histone modifications. Our findings reveal that the HDA6-MSI1 module mediates the interaction between histone H3 deacetylation and H3K27Me3 to repress gene expression involved in flowering time control.
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