Journal
PLANT JOURNAL
Volume 109, Issue 6, Pages 1355-1374Publisher
WILEY
DOI: 10.1111/tpj.15646
Keywords
population; bulk segregation analysis (BSA); next-generation sequencing (NGS); bulked segregant RNA-seq (BSR-seq); mapping algorithm; threshold; fine mapping
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This paper provides an overview of the development and application of Bulk Segregation Analysis (BSA) in the past decade, as well as a summary of related strategies for QTL fine mapping in combination with BSA.
Bulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative trait locus (QTL) mapping. With the development of next-generation sequencing (NGS) technology in the past 10 years, BSA methods and technical systems have been gradually developed and improved. At the same time, the ever-decreasing costs of sequencing accelerate NGS-based BSA application in different species, including eukaryotic yeast, grain crops, economic crops, horticultural crops, trees, aquatic animals, and insects. This paper provides a landscape of BSA methods and reviews the BSA development process in the past decade, including the sequencing method for BSA, different populations, different mapping algorithms, associated region threshold determination, and factors affecting BSA mapping. Finally, we summarize related strategies in QTL fine mapping combining BSA.
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