Journal
PLANT GROWTH REGULATION
Volume 96, Issue 2, Pages 237-241Publisher
SPRINGER
DOI: 10.1007/s10725-021-00771-z
Keywords
Rice seed; Endophytes; High-throughput screening; Antifungal activity; Biological control
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Funding
- Fundamental Research Funds for the Central Universities [FRF-TP-20-044A2, FRF-BR-19-003B, FRF-BR-20-03B, FRF-MP-20-39]
- Open Research Fund of State Key Laboratory of Hybrid Rice (Hunan Hybrid Rice Research Center) [2017KF04]
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This study developed a rapid, efficient one-step high-throughput method for screening endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice seeds. The technique allows for simultaneous screening and purification, identifying endophytic bacteria displaying antifungal activity and saving significant time.
Screening for microorganisms with antagonistic activity against crop pathogens is essential for microbial antagonist preparation, crop disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method for screening endophytic bacteria displaying antagonistic activity against Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that these bacteria could be directly screened from six sample groups using this method. This technique can achieve simultaneous screening and purification, identify endophytic bacteria displaying antifungal activity in rice seeds, and save significant time.
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