Journal
PLANT CELL REPORTS
Volume 41, Issue 1, Pages 139-151Publisher
SPRINGER
DOI: 10.1007/s00299-021-02792-4
Keywords
Celery; AgMYB12; Transcription factor; Overexpression; Apigenin
Categories
Funding
- Jiangsu Agricultural Science and Technology Innovation Fund [CX(18)2007]
- New Century Excellent Talents in University [NCET-11-0670]
- National Natural Science Foundation of China [31272175]
- Priority Academic Program Development of Jiangsu Higher Education Institutions Project (PAPD)
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The study identified that the overexpression of AgMYB12 in celery can enhance the accumulation of apigenin by interacting with the AgFNS gene, suggesting AgMYB12 as a positive regulator of apigenin biosynthesis.
Key message Overexpression of AgMYB12 in celery improved the accumulation of apigenin by interacting with the AgFNS gene. Celery is a common vegetable, and its essential characteristic is medicine food homology. A natural flavonoid and a major pharmacological component in celery, apigenin plays an important role in human health. In this study, we isolated a novel R2R3-MYB transcription factor that regulates apigenin accumulation from the celery cultivar 'Jinnan Shiqin' through yeast one-hybrid screening and designated it as AgMYB12. The AgMYB12 protein was located in the nucleus. It showed transcriptional activation activity and bound specifically to the promoter of AgFNS, a gene involved in apigenin biosynthesis. Phylogenetic tree analysis demonstrated that AgMYB12 belongs to the flavonoid branch. It contains two flavonoid-related motifs, SG7 and SG7-2, and shared a highly conserved R2R3 domain with flavonoid-related MYBs. The homologous overexpression of AgMYB12 induced the up-regulation of AgFNS gene expression and accumulation of apigenin and luteolin in celery. Additionally, the expression levels of apigenin biosynthesis-related genes, including AgPAL, AgCHI, AgCHS, Ag4CL, and AgC4H, increased in transgenic celery plants. These results indicated that AgMYB12 acted as a positive regulator of apigenin biosynthesis and activated the expression of AgFNS gene. The current study provides new information about the regulation mechanism of apigenin metabolism in celery and offers a strategy for cultivating the plants with high apigenin content.
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