Journal
PLANT BIOTECHNOLOGY REPORTS
Volume 17, Issue 2, Pages 255-273Publisher
SPRINGER
DOI: 10.1007/s11816-022-00741-5
Keywords
Aloesone synthase; Heterologous expression; Molecular docking; Promoter analysis; qRT-PCR expression; Rheum australe; Southern blotting
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Rheum australe, an endangered medicinal and vegetable herb, contains bio-active phyto-constituents with remedying properties. In this study, the RaALS gene, involved in the biosynthesis of these phyto-constituents, was isolated and characterized. The expression of RaALS was found to be correlated with the concentration of metabolites in different parts of the plant. Moreover, RaALS was also responsive to abiotic stressors. These findings highlight the importance of RaALS in the metabolic engineering of R. australe.
Rheum australe (Himalayan Rhubarb, Polygonaceae), an endangered medicinal and vegetable herb owes its age-old remedying properties to the bio-active phyto-constituents viz. anthraquinones, stilbenoids, chromones and dietary flavonoids. Polyketide pathway primarily involving the intricate Type III polyketide synthases (PKSs) contributes to the biosynthesis of these phyto-constituents. In the present study, we perform a homology-based approach to isolate an 1176 bp full-length cds sequence of the RaALS gene showing an equitable level of sequence similarity to related Type III PKSs at both nucleic acid and amino acid levels. In silico characterization revealed the presence of highly conserved amino acid residues found in nearly all Type III PKSs including the conserved active-site residues, signature motif and cyclization pocket residues with an exception of Ile256 and Gly258. Docking studies established major interactions between the starter acetyl-CoA and RaALS. Copy number analysis suggested slender evolution in Type III PKS in R. australe having a single copy of RaALS gene. qRT-PCR analyses revealed corroboration between the higher expression of RaALS in leaves followed by stem and root with that of the metabolite concentration. Expression studies further showed a direct increase of RaALS transcripts with the growing metabolite accretion in relation to altitude suggesting a probable involvement of specific Type III PKS in biosynthesis of the major phyto-constituents. Furthermore, abiotic stressors viz. methyl jasmonate, salicylic acid and UV light enhanced RaALS transcription hinting towards its role in defense mechanism in R. australe and highlighting the significance of RaALS as a prospective target for metabolic engineering.
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