Journal
PLANT BIOLOGY
Volume 24, Issue 2, Pages 344-355Publisher
WILEY
DOI: 10.1111/plb.13373
Keywords
functional characteristics; iron deficiency; Malus halliana
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Funding
- National Natural Science Foundation of China [31960581]
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This study identified 29 MhR2R3-MYB transcription factors in response to iron deficiency stress in Malus halliana, with further focus on MhR2R3-MYB4 which showed significant upregulation. Overexpression of MhR2R3-MYB4 enhanced tolerance to iron deficiency in Arabidopsis thaliana and led to multiple biochemical changes, suggesting its potential role in improving iron deficiency tolerance in apple.
Iron (Fe) is an essential element for plant growth and development. Fe deficiency can trigger leaf chlorosis and reduce fruit yield. Therefore, it is necessary to explore transcription factors in response to Fe deficiency stress. A total of 29 MhR2R3-MYB transcription factors were identified based on the transcriptome of Malus halliana under Fe deficiency stress. A comprehensive analysis of physical and chemical properties, gene structures, conserved motif composition, evolutionary relationship and chromosome distribution was performed. Subsequently, based on the transcriptome, 14 genes with the most significant expression under Fe deficiency stress were screened for qRT-PCR verification. Among them,the functional characteristics of MhR2R3-MYB4 (MD05G1089600) were further studied in Arabidopsis thaliana. Expression of 13 out of these 14 genes was upregulated, only one was downregulated. Maximum upregulation of MhR2R3-MYB4 under Fe deficiency was 36.39-fold and 58.21-fold compared with day 0 in leaves and roots, respectively. Overexpression of MhR2R3-MYB4 enhanced tolerance to Fe deficiency in A. thaliana and led to multiple biochemical changes: transgenic lines have higher chl a, chl b and Fe2+ content, higher enzyme activity (SOD, POD, CAT and FCR) and lower chlorosis than the wild type in Fe deficiency conditions. We suggest that MhR2R3-MYB4 plays an important part in Fe deficiency stress, which may contribute to improve Fe deficiency tolerance of apple in future.
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