Journal
PHYTOPATHOLOGIA MEDITERRANEA
Volume 60, Issue 3, Pages 403-407Publisher
MEDITERRANEAN PHYTOPATHOLOGICAL UNION
DOI: 10.36253/phyto-12709
Keywords
Potyvirus; Hungary; daffodil
Categories
Funding
- Hungarian Govern-ment
- European Social Fund
- Ministry for Innovation and Technology [TKP2020-IKA-12]
- [EFOP-3.6.1-16-2016-00006]
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In autumn 2017, autumn daffodil plants with yellow-green stripes on the leaves were observed at a botanical garden in Budapest, Hungary. Tests revealed that the symptomatic S. lutea leaf samples were infected with Narcissus late season yellows virus, marking the first report of this virus on Sternbergia lutea as a host.
In autumn 2017, autumn daffodil plants with yellow-green stripes on the leaves were observed at a botanical garden in Budapest, Hungary. Indicator plants were inoculated, but symptoms did not develop. RT-PCR tests of the indicator plants were also negative for the viruses. Potyvirus specific ACP-ELISA and RT-PCR were car-ried out on the symptomatic S. lutea leaf samples. RT-PCR with universal potyvirus primers resulted in one, approx. 1700 base pair PCR product. Phylogenetic analysis of the nucleotide sequence of the coat protein demonstrated 98.78-99.51% identity with three Japanese isolates of Narcissus late season yellows virus. While unidentified poty-virus infection of autumn daffodil has been previously reported, sequence data have not been published. Therefore, this is the first report of Sternbergia lutea as a host of Narcissus late season yellows virus.
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