Ammonium and nitrate affect sexually different responses to salt stress in Populus cathayana

Nitrogen (N) fertilization is a promising approach to improve salt tolerance. However, it is poorly known how plant sex and inorganic N alter salt stress-induced Na+ uptake, distribution and tolerance. This study employed Populus cathayana Rehder females and males to examine sex-related mechanisms of salt tolerance under nitrate (NO3-) and ammonium (NH4+) nutrition. Males had a higher root Na+ efflux, lower root-to-shoot translocation of Na+, and higher K+/Na+, which enhanced salt tolerance under both N forms compared to females. On the other hand, decreased root Na+ efflux and K+ retention, and an increased ratio of Na+ in leaves relative to shoots in females caused greater salt sensitivity. Females receiving NH4+ rather than NO3- had greater net root Na+ uptake, K+ efflux, and translocation to the shoots, especially in leaves. In contrast, males receiving NO3- rather than NH4+ had increased Na+ translocation to the shoots, especially in the bark, which may narrow the difference in leaf damage by salt stress between N forms despite a higher shoot Na+ accumulation and lower root Na+ efflux. Genes related to cell wall synthesis, K+ and Na+ transporters, and denaturized protein scavenging in the barks showed differential expression between females and males in response to salt stress under both N forms. These results suggested that the regulation of N forms in salt stress tolerance was sex-dependent, which was related to the maintenance of the K+/Na+ ratio in tissues, the ability of Na+- translocation to the shoots, and the transcriptional regulation of bark cell wall and proteolysis profiles.

Automated summary

This study revealed the gender-dependent regulation of salt stress tolerance by nitrogen forms, which was associated with the maintenance of the K+/Na+ ratio in tissues, the ability of Na+ translocation to the shoots, and the transcriptional regulation of bark cell wall and proteolysis profiles in response to salt stress under both nitrogen forms.