Journal
PARKINSONISM & RELATED DISORDERS
Volume 93, Issue -, Pages 77-84Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.parkreldis.2021.11.020
Keywords
microRNAs (miRNAs); Biomarkers; Parkinson's disease (PD)
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This study demonstrated the potential of exosomal miRNAs as biomarkers to differentiate between Parkinson's disease and Progressive Supranuclear Paralysis, with specific miRNAs like miR-21-3p, miR-22-3p, miR-223-5p, miR-425-5p, miR-21-3p, and miR-199a-5p showing promise in accurate discrimination. The classifier consisting of six exosomal miRNAs had high diagnostic sensitivity and specificity in distinguishing PSP from PD.
Introduction: Parkinson's disease (PD), a progressive neurodegenerative disease, can be misdiagnosed with atypical conditions such as Progressive Supranuclear Paralysis (PSP) due to overlapping clinical features. MicroRNAs (miRNAs) are small non-coding RNAs with a key role in post-transcriptional gene regulation. The aim was to identify a set of differential exosomal miRNAs biomarkers, which may aid in diagnosis. Methods: We analyzed the serum level of 188 miRNAs in a discovery set, by using RTqPCR based TaqMan assay, in a small cohort of healthy controls, PD and PSP patients. Subsequently, the differentially expressed miRNAs, between PSP and PD patients, were further tested in a larger and independent cohort of 33 healthy controls, 40 PD and 20 PSP patients. The most accurate diagnostic exosomal miRNAs classifiers were identified in a logistic regression model. Results: A statistically significant set of three exosomal miRNAs: miR-21-3p, miR-22-3p and miR-223-5p, discriminated PD from HC (area under the curve of 0.75), and a set of three exosomal miRNAs, miR-425-5p, miR-21-3p, and miR-199a-5p, discriminated PSP from PD with good diagnostic accuracy (area under the curve of 0.86). Finally, the classifier that best discriminated PSP from PD consisted of six exosomal miRNAs (area under the curve = 0.91), with diagnostic sensitivity and specificity of 0.89 and 0.90, respectively. Conclusions: Based on our analysis, these data showed that exosomal miRNAs could act as biomarkers to differentiate between PSP and PD.
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