Inductions of a CYP6 cluster conferring deltamethrin resistance in colonized and field-collected Culex pipiens pallens

Mosquitoes transmit many damaging vector-borne diseases. Unfortunately, the rise of insecticide resistance has become a major obstacle to mosquito control. A preliminary study showed that a CYP6 cluster is significant for deltamethrin resistance in colonized Culex pipiens pallens. Here, several field strains were collected to explore the association of the cluster in deltamethrin tolerance. We examined the effect of deltamethrin treatment on the cluster expression at a deltamethrin concentration of LC50 in these strains using five time points. As a result, both P450 induction and constitutive overexpression were associated with deltamethrin resistance. Deltamethrin could stimulate different expression sets in the P450 cluster in different strains, predominately correlated with the resistance level of the strain. Our results will offer more insight into working with the characterization of P450s related to insecticide resistance.

Automated summary

The CYP6 cluster plays a significant role in deltamethrin resistance, with different strains showing varying levels of resistance corresponding to different expression sets in the P450 cluster. This study provides valuable insights into understanding insecticide resistance mechanisms related to P450s.