Journal
PARASITOLOGY RESEARCH
Volume 121, Issue 4, Pages 1191-1198Publisher
SPRINGER
DOI: 10.1007/s00436-022-07426-6
Keywords
Schistosoma; NMR; Metabolomics; OPLS; Glutathione; ROS
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Funding
- CNR (National Research Council)-CNCCS (Collezione Nazionale di Composti Chimici e Centro di screening) Rare, Neglected and Poverty Related Diseases - Schistodiscovery Project [DSB.AD011.006]
- Ministero dell'Universita e della Ricerca PRIN [20154JRJPP_006]
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This article introduces the etiology of schistosomiasis, the way parasites acquire nutrients, and the optimal conditions for in vitro culture. The researchers used H-1-NMR spectroscopy to analyze the metabolic changes in S. mansoni under different culture conditions and discovered specific metabolic pathways modulated by different experimental conditions.
Schistosomiasis is a neglected tropical disease caused by parasitic flatworms (blood fluke) of the genus Schistosoma. Parasites acquire most nutrients for their development and sustainment within the definitive host either by ingestion into the gut or across the body surface. Over the years, the best conditions for long-term maintenance of parasites in vitro have been thoroughly established. In our hands, H-1-NMR spectroscopy represents a powerful tool to characterize the metabolic changes in S. mansoni in response to culturing condition perturbations. In order to compare the metabolic fingerprint of ex vivo and parasites cultured in vitro with or without the supplement of reduced glutathione, we conducted a pilot study applying the H-1-NMR spectroscopy-based metabolomics. We obtained new insight into specific metabolic pathways modulated under these different experimental conditions.
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