4.8 Article

Genome Mining of Aspergillus hancockii Unearths Cryptic Polyketide Hancockinone A Featuring a Prenylated 6/6/6/5 Carbocyclic Skeleton

Journal

ORGANIC LETTERS
Volume 23, Issue 22, Pages 8789-8793

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.1c03283

Keywords

-

Funding

  1. Australian Research Council [FT160100233, DP210102180, CRCPFIVE000119]
  2. Australian Research Council [FT160100233] Funding Source: Australian Research Council

Ask authors/readers for more resources

Activation of a cryptic polyketide synthase gene cluster hkn from Aspergillus hancockii by overexpressing the gene-cluster-specific transcription factor HknR led to the discovery of a novel polycyclic metabolite named hancockinone A. This compound, featuring a unique prenylated tetracyclic skeleton, exhibits moderate antibacterial activity and the role of cytochrome P450 HknE in constructing the five-membered ring in hancockinone A has been confirmed through heterologous expression, substrate feeding, and in vitro assays.
Activation of a cryptic polyketide synthase gene cluster hkn from Aspergillus hancockii via overexpression of the gene-cluster-specific transcription factor HknR led to the discovery of a novel polycyclic metabolite, which we named hancockinone A. The compound features an unprecedented prenylated 6/6/6/5 tetracarbocyclic skeleton and shows moderate antibacterial activity. Heterologous expression, substrate feeding, and in vitro assays confirmed the role of cytochrome P450 HknE in constructing the five-membered ring in hancockinone A from the precursor neosartoricin B.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available