Journal
NUCLEIC ACIDS RESEARCH
Volume 50, Issue 4, Pages 2302-2318Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac062
Keywords
-
Categories
Funding
- FONDECYT [1160176, 1190156, 1180798, 1211547, 11180621]
- CONICYT
Ask authors/readers for more resources
This study reveals the regulatory role of N-6-methyladenosine (m(6)A) deposition on HIV-1 full-length RNA packaging, as well as the interaction between HIV-1 Gag protein and RNA demethylase FTO. The results show that m(6)A deposition leads to increased Gag synthesis and reduced packaging, while FTO-mediated demethylation promotes the incorporation of the full-length RNA into viral particles.
During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, the mechanisms by which the HIV-1 Gag protein selects the two RNA molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate that deposition of N-6-methyladenosine (m(6)A) regulates full-length RNA packaging. While m(6)A deposition by METTL3/METTL14 onto the full-length RNA was associated with increased Gag synthesis and reduced packaging, FTO-mediated demethylation promoted the incorporation of the full-length RNA into viral particles. Interestingly, HIV-1 Gag associates with the RNA demethylase FTO in the nucleus and contributes to full-length RNA demethylation. We further identified two highly conserved adenosines within the 5 '-UTR that have a crucial functional role in m(6)A methylation and packaging of the full-length RNA. Together, our data propose a novel epitranscriptomic mechanism allowing the selection of the HIV-1 full-length RNA molecules that will be used as viral genomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available