4.8 Article

Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs

Related references

Note: Only part of the references are listed.
Review Biochemistry & Molecular Biology

Modifications of the human tRNA anticodon loop and their associations with genetic diseases

Jing-Bo Zhou et al.

Summary: Posttranscriptional modifications of tRNA, mainly catalyzed by enzymes and/or cofactors encoded by nuclear genes, play critical roles in maintaining tRNA structure and function complexity. Mutations of related genes are closely associated with diverse human diseases.

CELLULAR AND MOLECULAR LIFE SCIENCES (2021)

Article Biochemistry & Molecular Biology

Nucleotide resolution profiling of m3C RNA modification by HAC-seq

Jia Cui et al.

Summary: Cellular RNAs undergo various chemical modifications that regulate RNA expression and function, with dysregulation contributing to human disease. A novel technique called HAC-seq allows for precise mapping of m(3)C RNA modification. Analysis in human cells revealed tRNAs as the predominant RNA species modified by m(3)C.

NUCLEIC ACIDS RESEARCH (2021)

Review Biochemistry & Molecular Biology

The structural and functional workings of KEOPS

Jonah Beenstock et al.

Summary: KEOPS, a highly conserved five-subunit protein complex, plays crucial roles in cellular health and animal development. Research on KEOPS has provided important insights into its functions in cells and human diseases.

NUCLEIC ACIDS RESEARCH (2021)

Review Cell Biology

The expanding world of tRNA modifications and their disease relevance

Tsutomu Suzuki

Summary: tRNAs undergo heavy post-transcriptional modifications, which play crucial roles in accurate codon recognition and reading frame maintenance during protein synthesis. Recent studies have shown that tRNA modifications can be dynamically altered in response to cellular and environmental stresses, and deficiencies in tRNA modification can lead to mitochondrial diseases, neurological disorders, and cancer.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2021)

Article Biochemistry & Molecular Biology

Structure of a reaction intermediate mimic in t6A biosynthesis bound in the active site of the TsaBD heterodimer from Escherichia coli

Brett J. Kopina et al.

Summary: This study investigates the transfer mechanism of the key t(6)A modification in tRNA biosynthesis, focusing on the structure of the TsaBD heterodimer and its interaction with a phosphonate isosteric mimic of TC-AMP. The inhibitor binding to TsaBD via active site Zn atom suggests a potential oxyanion hole for catalysis, highlighting the metal's role as a binding scaffold for the intermediate. The phosphonate-bound crystal structure provides insights for the rational design of potent small molecule inhibitors for potential antibiotics.

NUCLEIC ACIDS RESEARCH (2021)

Article Biochemistry & Molecular Biology

Mutually exclusive substrate selection strategy by human m3C RNA transferases METTL2A and METTL6

Xue-Ling Mao et al.

Summary: This study revealed the presence of METTL2A and METTL2B in vivo, and successfully demonstrated their m(3)C32 modification activity on tRNA(Thr) and tRNA(ser)(GCU) in vitro. The research also found that G35 and t(6)A37 are essential for tRNA(Thr) m(3)C32 formation, while the anticodon loop and long arm are crucial for tRNA(ser)(GCU) m(3)C32 biogenesis.

NUCLEIC ACIDS RESEARCH (2021)

Article Biochemistry & Molecular Biology

Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins

Adrian Gabriel Torres et al.

Summary: The modification of adenosine to inosine at the 134th position of tRNA anticodons is essential for the translation of specific low-complexity proteins in eukaryotes, which require the cognate codons translated by 134-tRNAs for their synthesis.

NUCLEIC ACIDS RESEARCH (2021)

Article Biochemistry & Molecular Biology

Molecular basis for t6A modification in human mitochondria

Jing-Bo Zhou et al.

NUCLEIC ACIDS RESEARCH (2020)

Article Multidisciplinary Sciences

A substrate binding model for the KEOPS tRNA modifying complex

Jonah Beenstock et al.

NATURE COMMUNICATIONS (2020)

Article Biochemistry & Molecular Biology

The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase

Qi-Yu Zeng et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Biochemistry & Molecular Biology

Matching tRNA modifications in humans to their known and predicted enzymes

Valerie de Crecy-Lagard et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Biochemistry & Molecular Biology

Conformational communication mediates the reset step in t6A biosynthesis

Amit Luthra et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Multidisciplinary Sciences

Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome

Christelle Arrondel et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Structure and mechanism of a bacterial t6A biosynthesis system

Amit Luthra et al.

NUCLEIC ACIDS RESEARCH (2018)

Article Biochemistry & Molecular Biology

The structure of the TsaB/TsaD/TsaE complex reveals an unexpected mechanism for the bacterial t6A tRNA-modification

Sophia Missoury et al.

NUCLEIC ACIDS RESEARCH (2018)

Article Multidisciplinary Sciences

CO2-sensitive tRNA modification associated with human mitochondrial disease

Huan Lin et al.

NATURE COMMUNICATIONS (2018)

Article Biochemistry & Molecular Biology

tRNA N6-adenosine threonylcarbamoyltransferase defect due to KAE1/TCS3 (OSGEP) mutation manifest by neurodegeneration and renal tubulopathy

Simon Edvardson et al.

EUROPEAN JOURNAL OF HUMAN GENETICS (2017)

Article Genetics & Heredity

Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly

Daniela A. Braun et al.

NATURE GENETICS (2017)

Article Biochemistry & Molecular Biology

GtRNAdb 2.0: an expanded database of transfer RNA genes identified in complete and draft genomes

Patricia P. Chan et al.

NUCLEIC ACIDS RESEARCH (2016)

Article Cell Biology

Global translational impacts of the loss of the tRNA modification t(6)A in yeast

Patrick C. Thiaville et al.

MICROBIAL CELL (2016)

Article Biochemistry & Molecular Biology

Essentiality of threonylcarbamoyladenosine (t6A), a universal tRNA modification, in bacteria

Patrick C. Thiaville et al.

MOLECULAR MICROBIOLOGY (2015)

Article Biochemistry & Molecular Biology

Diversity of the biosynthesis pathway for threonylcarbamoyladenosine (t6A), a universal modification of tRNA

Patrick C. Thiaville et al.

RNA BIOLOGY (2014)

Article Biochemistry & Molecular Biology

In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya

Ludovic Perrochia et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemistry & Molecular Biology

Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells

Kazunori Watanabe et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemistry & Molecular Biology

Functional assignment of KEOPS/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification

Ludovic Perrochia et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemistry & Molecular Biology

Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system

Leo C. K. Wan et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Biochemistry & Molecular Biology

Biosynthesis of Threonylcarbamoyl Adenosine (t6A), a Universal tRNA Nucleoside

Christopher Deutsch et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2012)

Review Genetics & Heredity

Human Mitochondrial tRNAs: Biogenesis, Function, Structural Aspects, and Diseases

Tsutomu Suzuki et al.

ANNUAL REVIEW OF GENETICS, VOL 45 (2011)

Article Biochemistry & Molecular Biology

The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A

Madhusudhan Srinivasan et al.

EMBO JOURNAL (2011)

Article Biochemistry & Molecular Biology

A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification

Basma El Yacoubi et al.

EMBO JOURNAL (2011)

Article Medicine, Research & Experimental

Deficit of tRNALys modification by Cdkal1 causes the development of type 2 diabetes in mice

Fan-Yan Wei et al.

JOURNAL OF CLINICAL INVESTIGATION (2011)

Article Biochemistry & Molecular Biology

Strategies for the structural analysis of multi-protein complexes: Lessons from the 3D-Repertoire project

B. Collinet et al.

JOURNAL OF STRUCTURAL BIOLOGY (2011)

Article Biochemistry & Molecular Biology

The Sua5 Protein Is Essential for Normal Translational Regulation in Yeast

Changyi A. Lin et al.

MOLECULAR AND CELLULAR BIOLOGY (2010)

Article Biochemistry & Molecular Biology

tRNAdb 2009: compilation of tRNA sequences and tRNA genes

Frank Juehling et al.

NUCLEIC ACIDS RESEARCH (2009)

Article Biochemistry & Molecular Biology

The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA

Basma El Yacoubi et al.

NUCLEIC ACIDS RESEARCH (2009)

Article Biochemistry & Molecular Biology

The CP2 Domain of Leucyl-tRNA Synthetase Is Crucial for Amino Acid Activation and Post-transfer Editing

Xiao-Long Zhou et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2008)

Article Biochemistry & Molecular Biology

Atomic Structure of the KEOPS Complex: An Ancient Protein Kinase-Containing Molecular Machine

Daniel Y. L. Mao et al.

MOLECULAR CELL (2008)

Article Biochemistry & Molecular Biology

The role of modifications in codon discrimination by tRNALys UUU

FV Murphy et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2004)