Journal
NEW PHYTOLOGIST
Volume 233, Issue 5, Pages 2127-2143Publisher
WILEY
DOI: 10.1111/nph.17934
Keywords
cold stress; light signaling; myo-inositol; SlFHY3; Solanum lycopersicum
Categories
Funding
- National Key Research and Development Program of China [2018YFD1000800, 2019YFD1000300]
- National Natural Science Foundation of China [32122081, 31801904, 31991184]
- Liao Ning Revitalization Talents Program [XLYC1807020]
- Young and Middle-aged Science and Technology Innovation Talent Support Program in Shenyang [RC200449]
Ask authors/readers for more resources
A study reveals that under low red: far-red ratio (LR:FR) light conditions, cold stress induces the transcription of FAR-RED ELONGATED HYPOCOTYL3 (SlFHY3) in tomato plants. SlFHY3 physically interacts with ELONGATED HYPOCOTYL5 (SlHY5) to enhance the transcriptional activity of SlHY5 on MYO-INOSITOL-1-PHOSPHATE SYNTHASE 3 (SlMIPS3) and induce myo-inositol accumulation. Knocking out SlFHY3 increases cold susceptibility in tomato plants, while overexpressing SlFHY3 enhances cold tolerance.
Plants have evolved sophisticated regulatory networks to cope with dynamically changing light and temperature environments during day-night and seasonal cycles. However, the integration mechanisms of light and low temperature remain largely unclear. Here, we show that low red : far-red ratio (LR : FR) induces FAR-RED ELONGATED HYPOCOTYL3 (SlFHY3) transcription under cold stress in tomato (Solanum lycopersicum). Reverse genetic approaches revealed that knocking out SlFHY3 decreases myo-inositol accumulation and increases cold susceptibility, whereas overexpressing SlFHY3 induces myo-inositol accumulation and enhances cold tolerance in tomato plants. SlFHY3 physically interacts with ELONGATED HYPOCOTYL5 (SlHY5) to promote the transcriptional activity of SlHY5 on MYO-INOSITOL-1-PHOSPHATE SYNTHASE 3 (SlMIPS3) and induce myo-inositol accumulation in tomato plants under cold stress. Disruption of SlHY5 and SlMIPS3 largely suppresses the cold tolerance of SlFHY3-overexpressing plants and myo-inositol accumulation in tomato. Furthermore, silencing of SlMIPS3 drastically reduces myo-inositol accumulation and compromises LR : FR-induced cold tolerance in tomato. Together, our results reveal a crucial role of SlFHY3 in LR : FR-induced cold tolerance in tomato and unravel a novel regulatory mechanism whereby plants integrate dynamic environmental light signals and internal cues (inositol biosynthesis) to induce and control cold tolerance in tomato plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available