Journal
NEURO-ONCOLOGY
Volume 24, Issue 7, Pages 1056-1070Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/noab288
Keywords
DNA damage repair; glioblastoma; glioma stem cell; radioresistance
Categories
Funding
- National Natural Science Foundation of China [81872049, 81803053, 82003220, 81373201]
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This study presents a novel preclinical model for radiobiological studies and identifies a radiation-tolerant persister subpopulation that drives radioresistance by activating DNA damage repair and promoting stemness. The NF-kappa B-YY1-miR-103a regulatory axis is essential for the function of the radiation-tolerant persister cells. Furthermore, transferrin-functionalized nanoparticles improve radiosensitivity and provide a significant survival benefit.
Background Compelling evidence suggests that glioblastoma (GBM) recurrence results from the expansion of a subset of tumor cells with robust intrinsic or therapy-induced radioresistance. However, the mechanisms underlying GBM radioresistance and recurrence remain elusive. To overcome obstacles in radioresistance research, we present a novel preclinical model ideally suited for radiobiological studies. Methods With this model, we performed a screen and identified a radiation-tolerant persister (RTP) subpopulation. RNA sequencing was performed on RTP and parental cells to obtain mRNA and miRNA expression profiles. The regulatory mechanisms among NF-kappa B, YY1, miR-103a, XRCC3, and FGF2 were investigated by transcription factor activation profiling array analysis, chromatin immunoprecipitation, western blot analysis, luciferase reporter assays, and the MirTrap system. Transferrin-functionalized nanoparticles (Tf-NPs) were employed to improve blood-brain barrier permeability and RTP targeting. Results RTP cells drive radioresistance by preferentially activating DNA damage repair and promoting stemness. Mechanistic investigations showed that continual radiation activates the NF-kappa B signaling cascade and promotes nuclear translocation of p65, leading to enhanced expression of YY1, the transcription factor that directly suppresses miR-103a transcription. Restoring miR-103a expression under these conditions suppressed the FGF2-XRCC3 axis and decreased the radioresistance capability. Moreover, Tf-NPs improved radiosensitivity and provided a significant survival benefit. Conclusions We suggest that the NF-kappa B-YY1-miR-103a regulatory axis is indispensable for the function of RTP cells in driving radioresistance and recurrence. Thus, our results identified a novel strategy for improving survival in patients with recurrent/refractory GBM.
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