Journal
NEURO-ONCOLOGY
Volume 24, Issue 8, Pages 1230-1242Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/neuonc/noac001
Keywords
GLI signaling; glioma invasion; scaffold proteins; Shh signaling; tumor stem cells
Categories
Funding
- National Institutes of Health [5R21NS106537]
- Debbie's Brain Cancer Research Fund
- George W. Perkins III Research Fund
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DLG5 is upregulated in malignant gliomas and promotes invasion, viability, and self-renewal of glioblastoma stem cells. It regulates Sonic Hedgehog (Shh) signaling, making DLG5-deficient cells insensitive to Shh ligand, and its loss increases the proteasomal degradation of Gli1, leading to tumor stem cell sensitization. These findings reveal a novel and pro-tumoral role for DLG5 in glioblastoma, distinct from its proposed tumor-suppressor role in other solid tumors.
Background Tumor invasion, a hallmark of malignant gliomas, involves reorganization of cell polarity and changes in the expression and distribution of scaffolding proteins associated with polarity complexes. The scaffolding proteins of the DLG family are usually downregulated in invasive tumors and regarded as tumor suppressors. Despite their important role in regulating neurodevelopmental signaling, the expression and functions of DLG proteins have remained almost entirely unexplored in malignant gliomas. Methods Western blot, immunohistochemistry, and analysis of gene expression were used to quantify DLG members in glioma specimens and cancer datasets. Over-expression and knockdown of DLG5, the highest-expressed DLG member in glioblastoma, were used to investigate its effects on tumor stem cells and tumor growth. qRT-PCR, Western blotting, and co-precipitation assays were used to investigate DLG5 signaling mechanisms. Results DLG5 was upregulated in malignant gliomas compared to other solid tumors, being the predominant DLG member in all glioblastoma molecular subtypes. DLG5 promoted glioblastoma stem cell invasion, viability, and self-renewal. Knockdown of this protein in vivo disrupted tumor formation and extended survival. At the molecular level, DLG5 regulated Sonic Hedgehog (Shh) signaling, making DLG5-deficient cells insensitive to Shh ligand. Loss of DLG5 increased the proteasomal degradation of Gli1, underlying the loss of Shh signaling and tumor stem cell sensitization. Conclusions The high expression and pro-tumoral functions of DLG5 in glioblastoma, including its dominant regulation of Shh signaling in tumor stem cells, reveal a novel role for this protein that is strikingly different from its proposed tumor-suppressor role in other solid tumors.
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