Small proteins: overcoming size restrictions

Article Microbiology

Identification of Novel Translated Small Open Reading Frames in Escherichia coli Using Complementary Ribosome Profiling Approaches

Anne Stringer et al.

Summary: Small proteins of <51 amino acids are abundant across all domains of life, but they are often overlooked due to their small size and difficulty in prediction and detection. This study combines ribosome profiling data and drug treatments to identify hundreds of novel putative ORFs encoding short proteins in Escherichia coli. These newly discovered ORFs are mostly short and likely encode unstable proteins, with most of them not under purifying selection.

JOURNAL OF BACTERIOLOGY (2022)

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Article Biochemical Research Methods

Simultaneous ribosome profiling of hundreds of microbes from the human microbiome

Brayon J. Fremin et al.

Summary: MetaRibo-Seq is a ribosome profiling protocol designed for studying translation in the human fecal microbiome. It addresses challenges related to fecal sample handling, and provides a computational workflow for quality control and data analysis. This method allows researchers in standard molecular biology laboratories to study translation in the fecal microbiome within approximately 5 days.

NATURE PROTOCOLS (2021)

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Article Genetics & Heredity

Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach

Stephan Fuchs et al.

Summary: This study presents an optimized bioinformatics workflow for bacterial proteogenomics, improving the accuracy and reliability of genome annotations for small proteins. A significant number of small proteins were identified in Staphylococcus aureus Newman, including novel ones and highly conserved ones.

PLOS GENETICS (2021)

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Article Biochemistry & Molecular Biology

Ribosome profiling in archaea reveals leaderless translation, novel translational initiation sites, and ribosome pausing at single codon resolution

Diego Rivera Gelsinger et al.

NUCLEIC ACIDS RESEARCH (2020)

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Article Biochemistry & Molecular Biology

Are Antisense Proteins in Prokaryotes Functional?

Zachary Ardern et al.

FRONTIERS IN MOLECULAR BIOSCIENCES (2020)

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Small proteins: overcoming size restrictions

Related references

Identification of Novel Translated Small Open Reading Frames in Escherichia coli Using Complementary Ribosome Profiling Approaches

Simultaneous ribosome profiling of hundreds of microbes from the human microbiome

Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach

Ribosome profiling in archaea reveals leaderless translation, novel translational initiation sites, and ribosome pausing at single codon resolution

Are Antisense Proteins in Prokaryotes Functional?

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