4.8 Article

Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers

Journal

NATURE METHODS
Volume 18, Issue 10, Pages 1247-+

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41592-021-01261-w

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Funding

  1. Medical Research Council Funding Source: Medline
  2. EPSRC [EP/T03419X/1] Funding Source: UKRI

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Dynamic mass photometry allows label-free tracking and mass measurement of individual membrane-associated proteins diffusing on supported lipid bilayers. The approach can be used to monitor dynamic (dis)assembly of protein complexes.
The quantification of membrane-associated biomolecular interactions is crucial to our understanding of various cellular processes. State-of-the-art single-molecule approaches rely largely on the addition of fluorescent labels, which complicates the quantification of the involved stoichiometries and dynamics because of low temporal resolution and the inherent limitations associated with labeling efficiency, photoblinking and photobleaching. Here, we demonstrate dynamic mass photometry, a method for label-free imaging, tracking and mass measurement of individual membrane-associated proteins diffusing on supported lipid bilayers. Application of this method to the membrane remodeling GTPase, dynamin-1, reveals heterogeneous mixtures of dimer-based oligomers, oligomer-dependent mobilities, membrane affinities and (dis)association of individual complexes. These capabilities, together with assay-based advances for studying integral membrane proteins, will enable the elucidation of biomolecular mechanisms in and on lipid bilayers. Dynamic mass photometry allows label-free tracking and mass measurement of individual membrane-associated proteins diffusing on supported lipid bilayers. The approach can be used to monitor dynamic (dis)assembly of protein complexes.

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