Journal
INFECTION GENETICS AND EVOLUTION
Volume 41, Issue -, Pages 56-62Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.meegid.2016.03.022
Keywords
Trypanozoon; PCR-SSCP; ITS; Phylogenetic tree; Haplotype network
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Funding
- National Sciences Foundation of China [31272305, 31472058, 31301876, 31402029]
- Guangzhou Science Technology and Innovation Commission [201506010011]
- Natural Science Foundation of Guangdong Province [2014A030313164, 2015A030311042]
- Fundamental Research Funds for the Central University [141gpy23]
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The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei. (C) 2016 Elsevier B.V. All rights reserved.
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