4.8 Article

Widespread occurrence of covalent lysine-cysteine redox switches in proteins

Journal

NATURE CHEMICAL BIOLOGY
Volume 18, Issue 4, Pages 368-+

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41589-021-00966-5

Keywords

-

Funding

  1. Max-Planck Society
  2. DFG-funded Gottingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences GGNB

Ask authors/readers for more resources

This study reveals the presence of a lysine-cysteine redox switch in various proteins across different domains of life. These switches play important roles in enzyme catalysis and substrate binding in many proteins, linking lysine chemistry and redox biology.
We recently reported the discovery of a lysine-cysteine redox switch in proteins with a covalent nitrogen-oxygen-sulfur (NOS) bridge. Here, a systematic survey of the whole protein structure database discloses that NOS bridges are ubiquitous redox switches in proteins of all domains of life and are found in diverse structural motifs and chemical variants. In several instances, lysines are observed in simultaneous linkage with two cysteines, forming a sulfur-oxygen-nitrogen-oxygen-sulfur (SONOS) bridge with a trivalent nitrogen, which constitutes an unusual native branching cross-link. In many proteins, the NOS switch contains a functionally essential lysine with direct roles in enzyme catalysis or binding of substrates, DNA or effectors, linking lysine chemistry and redox biology as a regulatory principle. NOS/SONOS switches are frequently found in proteins from human and plant pathogens, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and also in many human proteins with established roles in gene expression, redox signaling and homeostasis in physiological and pathophysiological conditions. A survey of protein structures identifies widespread lysine-cysteine cross-links in functionally diverse proteins across all domains of life and in various structural motifs, where these redox switches control enzyme catalysis and/or ligand binding.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available