Journal
NANOTECHNOLOGY
Volume 33, Issue 7, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/1361-6528/ac3860
Keywords
Balangu; lallemantia royleana; PVA; mucilage; electrospinning
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The study investigates the synthesis of Balangu seed mucilage (BSM) combined with polyvinyl alcohol (PVA) to produce 3D electrospun cell culture scaffolds. The addition of PVA facilitated the formation of BSM/PVA nanofibers. Different BSM/PVA ratios were explored to achieve the desired structure for cell proliferation. Physiochemical assays and thermal analysis confirmed the positive effect of PVA incorporation on the samples. The biocompatibility of the scaffolds was verified through a cell culture experiment.
Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds. Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers. Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation. It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of approximate to 180 nm presents the most desirable scaffold structure for cell growth. The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests. According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples. Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected. The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds. The MTT assay is employed to verify the biocompatibility of the scaffolds. The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
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