Journal
NANOSCALE
Volume 14, Issue 10, Pages 3865-3877Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1nr06676e
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [12002292, 32071394]
- Fundamental Research Funds for the Central Universities [2682021ZTPY006]
- Analytical and Testing Center of Southwest Jiaotong University
Ask authors/readers for more resources
Biomimetic piezoelectric scaffolds with gradient piezoelectric properties were fabricated to induce the integrated osteochondral differentiation of rat MSCs. The piezoelectric potential positively modulated cell adhesion, intracellular calcium transients, Ca2+ binding proteins, and differentiation-related genes. The scaffold integrated osteogenic and chondrogenic differentiation on different areas, providing a smooth transition between cartilage and bone.
Biomimetic piezoelectric scaffolds provide a noninvasive method for in vivo cell regulation and tissue regeneration. Herein, considering the gradually varied piezoelectric properties of native cartilage and bone tissues, we fabricated biomimetic electrospun poly(l-lactic acid) (PLLA) nanofibrous mats with gradient piezoelectric properties to induce the integrated osteochondral differentiation of rat mesenchymal stem cells (MSCs). Nanofibrous mats are polarized under electric fields with linear variation of strength to generate gradient piezoelectricity, and cell adhesion-derived contraction forces could produce gradient piezoelectric potential on the scaffolds. Our results demonstrated that the piezoelectric potential could positively modulate cell adhesion, intracellular calcium transients, Ca2+ binding proteins, and differentiation-related genes. In addition, the differentiation of MSCs into osteogenic and chondrogenic lineages was integrated on a single scaffold at different areas with relatively high and low piezoelectricity values, respectively. The continuous gradient scaffold exhibited the potential to provide a smooth transition between the cartilage and bone, offering new insights to probe the regeneration mechanisms of the osteochondral tissue in a single scaffold and inspiring a future efficient and rational design of piezoelectric smart biomaterials for tissue engineering.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available