Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 39, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.nano.2021.102463
Keywords
Influenza; Recombinant vaccine; M2e peptide; Hemagglutinin; Nanoparticle
Funding
- Russian Foundation for Basic Research and Moscow City Government [21-3470038]
- Ministry of Science and Higher Education of the Russian Federation
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In this study, recombinant mosaic proteins containing tandem copies of M2e and HA2 were developed and characterized. These proteins self-assembled into nanoparticles with the inclusion of artificial self-assembling peptide (SAP). Intranasal immunization with these nanoparticles induced a strong immune response and high protection against different subtypes of influenza A virus.
The extracellular domain of the M2 protein (M2e) and conserved region of the second subunit of the hemagglutinin (HA2) could be used for the development of broad-spectrum vaccines against influenza A. Here we obtained and characterized recombinant mosaic proteins containing tandem copies of M2e and HA2 fused to an artificial self-assembling peptide (SAP). The inclusion of SAP peptides in the fusion proteins enabled their self-assembly in vitro into spherical particles with a size of 30-50 nm. Intranasal immunization of mice with these particles without additional adjuvants induced strong humoral immune response against M2e and the whole virus. Particles carrying both M2e and HA2 induced antigen-specific multifunctional CD4+ effector memory T cells. Immunization provided high protection of mice against the lethal challenge with different subtypes of influenza A virus. The obtained self-assembling nanoparticles can be used to develop a universal influenza vaccine. (c) 2021 Elsevier Inc. All rights reserved.
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