Journal
MOLECULES
Volume 27, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/molecules27020451
Keywords
bone sialoprotein; osteoblast; osterix; pamidronate; synergism; tannic acid
Funding
- Ministry of Higher Education Malaysia under Fundamental Fundamental Research Grant Scheme [FRGS/1/2020/STG01/USM/02/16]
Ask authors/readers for more resources
This study investigates the effect of combining tannic acid with pamidronate on human osteoblast cells. The combination treatment showed synergistic effects, promoting osteoblast proliferation and mineralization, as well as increasing the expression of bone-specific proteins and genes.
Background: This study investigates the effect of tannic acid (TA) combined with pamidronate (PAM) on a human osteoblast cell line. Methods: EC50 for TA, PAM, and different combination ratios of TA and PAM (25:75, 50:50, 75:25) were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The combination index value was utilized to analyze the degree of drug interaction, while trypan blue assay was applied to analyze the cells proliferation effect. The mineralization and detection of bone BSP and Osx genes were determined via histochemical staining and PCR test, respectively. Results: The EC50 of osteoblasts treated with TA and a 75:25 ratio of TA and PAM were more potent with lower EC50 at 0.56 mu g/mL and 0.48 mu g/mL, respectively. The combination of TA and PAM (75:25) was shown to have synergistic interaction. On Day 7, both TA and PAM groups showed significantly increased proliferation compared with control and combination groups. On Day 7, both the TA and combination-treated groups demonstrated a higher production of calcium deposits than the control and PAM-treated groups. Moreover, on Day 7, the combination-treated group showed a significantly higher expression of BSP and Osx genes than both the TA and PAM groups. Conclusion: Combination treatment of TA and PAM at 75:25 ameliorated the highest enhancement of osteoblast proliferation and mineralization as well as caused a high expression of BSP and Osx genes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available