Journal
MOLECULES
Volume 26, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/molecules26226988
Keywords
myocardial infarction; cardiac troponin; cTnI-detection; calcein liposomes; fluorescence; signal quantification
Funding
- American University of Sharjah, UAE
- Open Access Program at the American University of Sharjah, UAE
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The study developed an efficient fluorescent sandwich immunoassay for sensing and quantifying serum cTnI in clinical settings. The assay showed sensitivity and selectivity towards cTnI, with a detection limit of 6.5 ng/mL in spiked human serum samples.
Cardiovascular diseases (CVDs) are one of the foremost causes of mortality in intensive care units worldwide. The development of a rapid method to quantify cardiac troponin I (cTnI)-the gold-standard biomarker of myocardial infarction (MI) (or heart attack )-becomes crucial in the early diagnosis and treatment of myocardial infarction (MI). This study investigates the development of an efficient fluorescent sandwich immunoassay using liposome-based fluorescent signal amplification and thereby enables the sensing and quantification of serum-cTnI at a concentration relevant to clinical settings. The calcein-loaded liposomes were utilized as fluorescent nano vehicles, and these have exhibited appropriate stability and efficient fluorescent properties. The standardized assay was sensitive and selective towards cTnI in both physiological buffer solutions and spiked human serum samples. The novel assay presented noble analytical results with sound dynamic linearity over a wide concentration range of 0 to 320 ng/mL and a detection limit of 6.5 ng/mL for cTnI in the spiked human serum.
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