4.6 Article

Dereplication and Quantification of Major Compounds of Convolvulus arvensis L. Extracts and Assessment of Their Effect on LPS-Activated J774 Macrophages

Journal

MOLECULES
Volume 27, Issue 3, Pages -

Publisher

MDPI
DOI: 10.3390/molecules27030963

Keywords

field bindweed; GNPS; molecular networking; HPLC-UV-HRMS; phenolics; inflammation; biological screening

Funding

  1. Higher Education Commission (HEC), Pakistan under the project Faculty Development Program of Bahauddin Zakariya University Multan (100 PhD foreign scholarships) (Prime Minister's directive)

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The anti-inflammatory potential of Convolvulus arvensis was evaluated and the results showed that the extracts and major constituents of the plant can significantly inhibit the expression of pro-inflammatory mediators, supporting its use in the treatment of inflammatory diseases.
Convolvulus arvensis is used in Pakistani traditional medicine to treat inflammation-related disorders. Its anti-inflammatory potential was evaluated on hexane, dichloromethane, ethyl acetate, methanol, and aqueous extracts of whole plant on pro-inflammatory mediators in LPS-activated murine macrophage J774 cells at the non-cytotoxic concentration of 50 mu g/mL. Ethyl acetate (ARE) and methanol (ARM) extracts significantly decreased mRNA levels of IL-6, TNF-alpha, MCP-1, COX-2, and iNOS. Furthermore, both extracts dose dependently decreased IL-6, TNF-alpha, and MCP-1 secretion. Forty-five compounds were putatively identified in ARE and ARM by dereplication (using HPLC-UV-HRMSn analysis and molecular networking), most of them are reported for the first time in C. arvensis, as for example, nineteen phenolic derivatives. Rutin, kaempferol-3-O-rutinoside, chlorogenic acid, 3,5-di-O-caffeoylquinic acid, N-trans-p-coumaroyl-tyramine, and N-trans-feruloyl-tyramine were main constituents identified and quantified by HPLC-PDA in ARE and ARM. Furthermore, chlorogenic acid, tyramine derivatives, and the mixture of the six identified major compounds significantly decreased IL-6 secretion by LPS-activated J774 cells. The activity of N-trans-p-coumaroyl-tyramine is shown here for the first time. Our results indicate that ARE, ARM and major constituents significantly inhibited the expression of pro-inflammatory mediators, which supports the use of this plant to treat inflammatory diseases.

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