Journal
MOLECULES
Volume 26, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/molecules26216564
Keywords
MDA-MB 231 cells; tumor-targeting bacteriophages; chemical modification of bacteriophages; FAM-NHS; theranostic drugs
Funding
- Russian Science Fund [19-44-02006]
- Russian Science Foundation [19-44-02006] Funding Source: Russian Science Foundation
Ask authors/readers for more resources
Tumor-targeting bacteriophages were successfully modified using FAM-NHS ester, with the maximum modification coefficient observed at 4mM concentration. The modified bacteriophage retained the ability to penetrate MDA-MB-231 cells, indicating the potential for producing tumor-targeting diagnostic and therapeutic agents. Optimizing phage modification conditions could be an effective strategy for developing theranostic drugs.
Background: Tumor-targeting bacteriophages can be used as a versatile new platform for the delivery of diagnostic imaging agents and therapeutic cargo. This became possible due to the development of viral capsid modification method. Earlier in our laboratory and using phage display technology, phages to malignant breast cancer cells MDA-MB 231 were obtained. The goal of this study was the optimization of phage modification and the assessment of the effect of the latter on the efficiency of phage particle penetration into MDA-MB 231 cells. Methods: In this work, we used several methods, such as chemical phage modification using FAM-NHS ester, spectrophotometry, phage amplification, sequencing, phage titration, flow cytometry, and confocal microscopy. Results: We performed chemical phage modification using different concentrations of FAM-NHS dye (0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM). It was shown that with an increase of the modification degree, the phage titer decreases. The maximum modification coefficient of the phage envelope with the FAM-NHS dye was observed with 4 mM modifying agent and had approximately 804,2 FAM molecules per phage. Through the immunofluorescence staining and flow cytometry methods, it was shown that the modified bacteriophage retains the ability to internalize into MDA-MB-231 cells. The estimation of the number of phages that could have penetrated into one tumor cell was conducted. Conclusions: Optimizing the conditions for phage modification can be an effective strategy for producing tumor-targeting diagnostic and therapeutic agents, i.e., theranostic drugs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available