4.6 Article

Novel Antimicrobial Peptides from a Cecropin-Like Region of Heteroscorpine-1 from Heterometrus laoticus Venom with Membrane Disruption Activity

Journal

MOLECULES
Volume 26, Issue 19, Pages -

Publisher

MDPI
DOI: 10.3390/molecules26195872

Keywords

cecropin; CeHS-1; antimicrobial peptide; sequences modification

Funding

  1. Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
  2. KKU Research Fund, fiscal year 2020
  3. KKU Research Fund, fiscal year 2021

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The rising prevalence of antimicrobial resistance is a severe health issue. The invention of new antimicrobial agents, such as antimicrobial peptides like Heteroscorpine-1, shows promise in combating bacterial strains. Mutants of CeHS-1 have potential to be developed as novel antimicrobial peptides with increased efficacy and reduced toxicity.
The increasing antimicrobial-resistant prevalence has become a severe health problem. It has led to the invention of a new antimicrobial agent such as antimicrobial peptides. Heteroscorpine-1 is an antimicrobial peptide that has the ability to kill many bacterial strains. It consists of 76 amino acid residues with a cecropin-like region in N-terminal and a defensin-like region in the C-terminal. The cecropin-like region from heteroscorpine-1 (CeHS-1) is similar to cecropin B, but it lost its glycine-proline hinge region. The bioinformatics prediction was used to help the designing of mutant peptides. The addition of glycine-proline hinge and positively charged amino acids, the deletion of negatively charged amino acids, and the optimization of the hydrophobicity of the peptide resulted in two mutant peptides, namely, CeHS-1 GP and CeHS-1 GPK. The new mutant peptide showed higher antimicrobial activity than the native peptide without increasing toxicity. The interaction of the peptides with the membrane showed that the peptides were capable of disrupting both the inner and outer bacterial cell membrane. Furthermore, the SEM analysis showed that the peptides created the pore in the bacterial cell membrane resulted in cell membrane disruption. In conclusion, the mutants of CeHS-1 had the potential to develop as novel antimicrobial peptides.

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