Journal
MOLECULES
Volume 26, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/molecules26226882
Keywords
schisandrin B; hepatic stellate cell; apoptosis; TGF-beta 1
Funding
- Scientific and Technologic Foundation of Jilin Province [20200708070YY]
- Central Public-interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences [125161034-2021-016, CAAS-ASTIP-ISAPS-2021-010]
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The study demonstrates that Schisandrin B inhibits the activity of hepatic stellate cells induced by TGF-beta 1 by promoting apoptosis.
The activation of hepatic stellate cells (HSC) plays a key role in the progression of hepatic fibrosis, it is essential to remove activated HSC through apoptosis to reverse hepatic fibrosis. Schisandrin B (Sch B) is the main chemical component of schisandrin lignan, and it has been reported to have good hepatoprotective effects. However, Schisandrin B on HSC apoptosis remains unclear. In our study, we stimulated the HSC-T6 and LX-2 cell lines with TGF-beta 1 to induce cell activation, and the proliferation and apoptosis of the activated HSC-T6 and LX-2 cells were detected after treatment with different doses of Schisandrin B. Flow cytometry results showed that Sch B significantly reduced the activity of activated HSC-T6 and LX-2 cells and significantly induced apoptosis. In addition, the cleaved-Caspase-3 levels were increased, the Bax activity was increased, and the Bcl-2 expression was decreased in HSC-T6 and LX-2 cells treated with Sch B. Our study showed that Sch B inhibited the TGF-beta 1-induced activity of hepatic stellate cells by promoting apoptosis.
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