4.7 Article

The establishment of CDK9/RNA PolII/H3K4me3/DNA methylation feedback promotes HOTAIR expression by RNA elongation enhancement in cancer

Journal

MOLECULAR THERAPY
Volume 30, Issue 4, Pages 1597-1609

Publisher

CELL PRESS
DOI: 10.1016/j.ymthe.2022.01.038

Keywords

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Funding

  1. General Research Fund, Research Grants Council of Hong Kong [CUHK462713, CUHK14102714, CUHK14171217, CUHK14120618, CUHK14120419]
  2. National Natural Science Foundation of China [81672323]
  3. CUHK

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Long non-coding RNA HOTAIR is overexpressed in multiple cancers and promotes tumor growth and metastasis. This study reveals that hypermethylation of intragenic exon CpG islands is correlated with HOTAIR expression, and methylation enhances the transcription elongation process of HOTAIR. Furthermore, targeting the oncogenic CDK7-CDK9-H3K4me3 axis downregulates HOTAIR expression and inhibits cell growth in many cancers.
Long non-coding RNA HOX Transcript Antisense RNA (HOTAIR) is overexpressed in multiple cancers with diverse genetic profiles. Importantly, since HOTAIR heavily contributes to cancer progression by promoting tumor growth and metastasis, HOTAIR becomes a potential target for cancer therapy. However, the underlying mechanism leading to HOTAIR deregulation is largely unexplored. Here, we performed a pan-cancer analysis using more than 4,200 samples and found that intragenic exon CpG island (Ex-CGI) was hypermethylated and was positively correlated to HOTAIR expression. Also, we revealed that ExCGI methylation promotes HOTAIR expression through enhancing the transcription elongation process. Furthermore, we linked up the aberrant intragenic tri-methylation on H3 at lysine 4 (H3K4me3) and Ex-CGI DNA methylation in promoting transcription elongation of HOTAIR. Targeting the oncogenic CDK7-CDK9-H3K4me3 axis downregulated HOTAIR expression and inhibited cell growth in many cancers. To our knowledge, this is the first time that a positive feedback loop that involved CDK9-mediated phosphorylation of RNA Polymerase II Serine 2 (RNA Poll Ser2), H3K4me3, and intragenic DNA methylation, which induced robust transcriptional elongation and heavily contributed to the upregulation of oncogenic lncRNA in cancer has been demonstrated. Targeting the oncogenic CDK7-CDK9-H3K4me3 axis could be a novel therapy in many cancers through inhibiting the HOTAIR expression.

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