Journal
MOLECULAR NEUROBIOLOGY
Volume 59, Issue 1, Pages 717-730Publisher
SPRINGER
DOI: 10.1007/s12035-021-02594-5
Keywords
Protease-activated receptor 2; Microglia; Neuron; Hypoxic-ischemic injury
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Funding
- National Natural Science Foundation of China [32070954, 82001167]
- Beijing Natural Science Foundation [7212066]
- Beijing Institute of Technology Research Fund Program [2020CX04166]
- Zhejiang Provincial Natural Science Foundation [Q20H140040]
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The study found that PAR2 expression was abundant in neurons but barely in microglia after hypoxia-ischemia injury. Conversely, PAR2 expression was barely in neurons but significantly increased in activated microglia. PAR2 activation mediated cross-talk between microglia and neurons, leading to inflammatory responses, neuronal damage, chemokine regulation, and phagocytosis control. Interventions targeting PAR2 may provide insights into inflammatory-related diseases.
Regulation of microglial activation and neuroinflammation are critical factors in the pathogenesis of ischemic brain injury. Interest in protease-activated receptor 2 (PAR2) as a pharmaceutical target for various diseases is creasing. However, it is unclear the expression and functions of PAR2 in hypoxia-ischemic (HI) brain injury. Mice with HI and cells with oxygen-glucose deprivation and reoxygenation (OGD/R) were studied. Immunoblot and qRT-PCR were used to study the differential gene expression in cultured microglia and neurons. Immunofluorescent staining was used to study the expression pattern of PAR2 in the HI brain and phagocytotic activity of microglia after OGD/R. In neonatal mice brain after HI, we found PAR2 expression was abundant in neurons, but barely in microglia from the contralateral side of cortex and hippocampus. Conversely, PAR2 expression was barely in neurons while significantly increased in activated microglia from the ipsilateral side of cortex and hippocampus. The activations of PAR2 were increased in both microglia and neuron in a cell model of OGD/R. PAR2 activation mediated the cross-talk between microglia and neurons including the following: microglial PAR2 mediated inflammatory responses that induced neuronal damage; neuronal PAR2 regulated chemokines that recruited activated microglia to damage area; microglia PAR2 controlled the phagocytosis of degenerating neurons. These data suggested differential expression and distinct roles of PAR2 in microglia and neurons after HI injury; thereby, interventions targeting PAR2 may provide insights into the inflammatory-related diseases.
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